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ARS Home » Midwest Area » Columbus, Ohio » Soil Drainage Research » Research » Publications at this Location » Publication #163807
Title: INTEGRATED GENOMIC AND METABOLIC ANALYSES OF HYPOXIC STRESS RESPONSE IN FLOODING-TOLERANT SAG12:IPT ARABIDOPSIS

Author
item LIU, FENGLONG - THE INST FOR GENOMIC RES.
item Vantoai, Tara
item KLAPA, MARIA - THE UNIV. OF MARYLAND
item MOY, LINDA - THE INST FOR GENOMIC RES.
item BLOCK, GEOFFREY - THE INST FOR GENOMIC RES,
item KANANI, HARIN - THE UNIV. OF MARYLAND
item LINFORD, LARA - THE INST FOR GENOMIC RES.
item QUACKENBUSH, JOHN - THE INST FOR GEONOMIC RES

Submitted to: American Society of Plant Biologists Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 5/20/2004
Publication Date: 7/24/2004
Citation: Liu, F., Vantoai, T.T., Klapa, M., Moy, L., Block, G., Kanani, H., Linford, L., Quackenbush, J. 2004. Integrated genomic and metabolic analyses of hypoxic stress response in flooding-tolerant sag12:ipt arabidopsis [abstract]. American Society of Plant Biologists Annual Meeting. Paper No. 748.

Interpretive Summary:

Technical Abstract: Plants have evolved adaptation mechanisms to sense changes in their environment and make adjustments in gene expression and metabolism to enhance their stress tolerance. To gain insight into how plants respond to conditions of low oxygen, we examined both wild-type and flooding-tolerant transgenic plants with autoregulated cytokinin synthesis, comparing gene transcript and cellular metabolite levels at 9 time points over 24 hours in plants grown in 20% oxygen (equivalent to ambient air) as well as those kept at 3% oxygen. Gene expression data were collected using whole-genome DNA amplicon microarrays representing the more than 27,000 predicted nuclear, chloroplastic, and mitochondrial genes; gas chromatography - mass spectrometry (GC-MS) was used to obtain profiles on hundreds of metabolites including sugars, sugar alcohols, amino acids, and organic acids. Analysis of expression data revealed statistically significant temporal patterns of gene expression. Genes involved in fermentation pathways (PDC, ADH1 and LDH1), sucrose utilization (SS), and C2H4 synthesis (ACC oxidase) were significantly up-regulated under conditions of low oxygen. Members of copper/zinc SODs and xyloglucan endotransglycosylase family are among those down-regulated genes in response to hypoxic stress. Association analysis is providing insight into the functions of those genes that were not previously known to be involved in hypoxic response and those genes of unknown function. Correlation of gene expression and metabolite profile data will potentially enable inferences into enzyme activities and dynamic metabolite fluxes along hypoxia-perturbed pathways.