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ARS Home » Southeast Area » Little Rock, Arkansas » Arkansas Children's Nutrition Center » Research » Publications at this Location » Publication #163959
Title: LC-MS/MS ANALYSIS OF LYSOPHOSPHOLIPIDS ASSOCIATED WITH SOY PROTEIN ISOLATE

Author
item FANG, NIANBAI - ACNC/UAMS
item YU, SHANGGONG - ACNC
item BADGER, THOMAS - ACNC/UAMS

Submitted to: Journal of Agricultural and Food Chemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/9/2003
Publication Date: 10/14/2003
Citation: Fang, N., Yu, S., Badger, T.M. 2003. Lc-ms/ms analysis of lysophospholipids associated with soy protein isolate. Journal of Agricultural and Food Chemistry. 51:6676-6682.

Interpretive Summary: Approximately 20-25% of babies consuming formula are fed formulas containing soy protein as the sole protein source. Isoflavone phytoestrogens are considered as major bioactive components present in soy protein isolate (SPI) and the most widely studied phytochemicals in human diets and soy infant formulas. In the present study, LC-MS/MS analysis of SPI phytochemical extract resulted in identification of fifty-six lysophospholipids (lyso-PLs). Lyso-PLs are important molecules involved in cell signaling and they are growth factors experimentally linked to cardiovascular disease, cancer and neurological disorders. These finding suggests that biological effects of PLs associated with SPI on infant clearly needs to be considered as part of an overall evaluation of potential health benefits. Our future work will include studies on these important compounds.

Technical Abstract: Approximately 20-25% of babies consuming formula are fed formulas containing soy protein as the sole protein source. Isoflavone phytoestrogens are considered as major bioactive components present in soy protein isolate (SPI) and the most widely studied phytochemicals in human diets and soy infant formulas. In the present study, LC-MS/MS analysis of SPI phytochemical extract resulted in identifications of fifty-six lysophospholipids (lyso-PL), including eighteen lysophosphatidylcholines (lyso-PC), twelve lysophosphatidylethanolamines (lyso-PE), eleven lysophosphatidylinositols (lyso-PI), eleven lysophosphatidic acids (lyso-PA) and four lysophosphatidylglycerols (lyso-PG). The LC-MS/MS conditions were first developed using commercially available standard mixtures. Under these conditions, lyso-PL compounds could be separated by LC and yielded unique fragments and neutral losses in the electrospray ionization mass spectrometry (ESI-MS) with both positive- and negative- ion modes, quite indicative of the polar headgroups for different subclasses. This is the first characterization of lyso-PL in SPI. Lyso-PLs are important cell signaling and growth factor molecules experimentally linked to cardiovascular function and disease, cancer, and neurological disorders. These findings suggest that biological effects of PLs associated with SPI in infants clearly needs to be considered as part of an overall evaluation of potential health benefits. Key words: Soy protein isolate (SPI); SPI phytochemical; lysophospholipids; LC-MS/MS