Author
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BEKAL, SADIA - UNIV OF ILLINOIS |
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ATIBALENTJA, NDEME - UNIV OF ILLINOIS |
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NIBLACK, TERRY - UNIV OF ILLINOIS |
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Noel, Gregory |
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SMYTH, CHARLES - UNIV OF ILLINOIS |
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LAMBERT, KRIS - UNIV OF ILLINOIS |
Submitted to: Nematology
Publication Type: Abstract Only Publication Acceptance Date: 4/19/2004 Publication Date: 8/7/2004 Citation: BEKAL, S., ATIBALENTJA, N., NIBLACK, T.L., NOEL, G.R., SMYTH, C.A., LAMBERT, K.N. 2004. Selection of HGCM-1 alleles of heterodera glycines on resistant soybeans. Nematology. Interpretive Summary: Technical Abstract: The soybean cyst nematode (SCN), Heterodera glycines, is the most destructive pathogen of soybean in the Unites States. Diversity in the parasitic ability of the nematode allows SCN to frequently grow on resistant soybean. Hgcm-1 is a SCN chorismate mutase, which is thought to function as a candidate virulence gene. Hgcm-1A and Hgcm-1B are two members of the Hgcm-1 gene family that have been isolated from avirulent and virulent inbred SCN. In this study, we conducted controlled crosses between two inbred SCN lines to generate a F2 SCN segregating population for Hgcm-1A and B. This population was then selected on resistant (PI88788, PI90763, Hartwig) and susceptible (Lee 74) soybeans and genotyped using a real-time PCR (QPCR) assay. The QPCR analysis showed that SCN growing on PI88788 were predominately Hgcm-1A type as a result of a statistically significant drop of Hgcm-1B allele in the population. No significant difference was observed in SCN population grown on other resistant soybeans compared to the susceptible plant. Hg-cm-1A and 1B genes are highly repetitive in the SCN genome. The observation that they segregate approximately in a 1:2:1 ratio indicates Hg-cm-1 consists of a block of tandemly arrayed genes that segregate as a single locus. |