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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Sunflower and Plant Biology Research » Research » Publications at this Location » Publication #164855
Title: ACETOHYDROXYACID SYNTHASE MUTATIONS ASSOCIATED WITH RESISTANCE TO IMIDAZOLINONE OR SULFONYLUREA HERBICIDES IN SUNFLOWER

Author
item KOLKMAN, JUDITH - OREGON STATE UNIV.
item SLABAUGH, MARY - OREGON STATE UNIV.
item BRUNIARD, JOSE - NORTH DAKOTA STATE UNIV.
item BERRY, SIMON - ADVANTA, NORFOLK, UK
item BUSHMAN, B SHAUN - OREGON STATE UNIV.
item OLUNGU, CHRISTINE - ADVANTA, TIENEN, BELGIUM
item MAES, NELE - ADVANTA, TIENEN, BELGIUM
item ABRATTI, GUSTAVO - ADVANTA, ARGENTINA
item ZAMBELLI, ANDRES - ADVANTA, ARGENTINA
item MILLER, JERRY
item LEON, ALBERTO - ADVANTA, ARGENTINA
item KNAPP, STEVEN - OREGON STATE UNIV.

Submitted to: Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/27/2004
Publication Date: 8/10/2004
Citation: Kolkman, J.M., Slabaugh, M.B., Bruniard, J.M., Berry, S., Bushman, B., Olungu, C., Maes, N., Abratti, G., Zambelli, A., Miller, J.F., Leon, A., Knapp, S.J. 2004. Acetohydroxyacid synthase mutations associated with resistance to imidazolinone or sulfonylurea herbicides in sunflower. Theoretical and Applied Genetics. 109:1147-1159.

Interpretive Summary: In 1996 a farmer in Kansas discovered wild sunflower plants that were resistant to the imidazolinone class herbicide Pursuit. Seeds were transferred to USDA-ARS in Fargo, ND, and crosses were made to cultivated plants. Two populations, IMISUN-1 and IMISUN-2, and the inbred line HA 425 were created and released to industry and public development programs. The objectives of this investigation were to isolate resistance genes in the released germplasm, identify mutations in the genes imparting resistance, and develop tools for marker assisted selection of herbicide resistance genes in sunflower. Three resistance genes were identified, cloned, and sequenced from the herbicide-resistant germplasms. Markers for the three genes were developed and genetically mapped to the chromosome linkage groups 2, 6, and 9, respectively. The C/T SNP marker in codon 205 of gene 1 was associated with the partially dominant resistance to the imidazolinone herbicides. The molecular breeding tools described in this investigation create the basis for rapidly identifying new mutations in the genes providing resistance and performing marker assisted selection for herbicide resistance in sunflower.

Technical Abstract: In 1996 a farmer in Kansas discovered wild sunflower (Helianthus annuus L.) plants that were resistant to the imidazolinone class herbicide Pursuit (imazethapyr). Seeds were transferred to USDA-ARS in Fargo, ND, and crosses were made to cultivated plants. Two populations, IMISUN-1 and IMISUN-2, and the inbred line HA 425 were created and released to industry and public development programs. The objectives of this investigation were to isolate resistance genes in the released germplasm, identify mutations in the genes imparting resistance, and develop tools for marker assisted selection of herbicide resistance genes in sunflower. Three resistance genes were identified, cloned, and sequenced from the herbicide-resistant germplasms. Markers for the three genes were developed and genetically mapped to the chromosome linkage groups 2, 6, and 9, respectively. The C/T SNP marker in codon 205 of gene 1 was associated with the partially dominant resistance to the imidazolinone herbicides. The molecular breeding tools described in this investigation create the basis for rapidly identifying new mutations in the genes providing resistance and performing marker assisted selection for herbicide resistance in sunflower.