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ARS Home » Midwest Area » Columbia, Missouri » Biological Control of Insects Research » Research » Publications at this Location » Publication #164941
Title: APPLICATION OF INTER-SIMPLE SEQUENCE REPEATS (ISSR) TO INSECT CELL LINES: IDENTIFICATION AT THE CLONAL AND TISSUE-SPECIFIC LEVEL

Author
item Grasela, James
item McIntosh, Arthur
item Goodman, Cynthia

Submitted to: Congress on In Vitro Biology
Publication Type: Abstract Only
Publication Acceptance Date: 5/14/2004
Publication Date: 5/17/2004
Citation: Grasela, J.J., Mcintosh, A.H., Goodman, C.L. 2003. Application of inter-simple sequence repeats (issr) to insect cell lines: identification at the clonal and tissue-specific level [abstract]. Congress on In Vitro Biology. 40:41A

Interpretive Summary:

Technical Abstract: Inter-simple sequence repeat (ISSR) primers designed to anneal to microsatellites were used to obtain DNA fingerprint profiles to distinguish between 16 established insect cell lines derived from an assortment of lepidopteran, dipteran, and coleopteran species. Three different levels of cell line comparison were made: (1) between parents and their clones; (2) between cell lines derived from different tissues from the same species; and (3) between cell lines derived from different insect species. Of the 16 repeat oligonucleotide primers employed in this study, nine primers generated several unique markers to distinguish between parental cell lines and their clones. Four of the 16 primers also generated DNA profiles with a number of unique bands, enabling the distinction between cell lines derived from specific tissues from the same species. In addition, ISSR-generated DNA profiles provided the greatest number of unique markers to easily distinguish between insect cell lines derived from different species.