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ARS Home » Research » Publications at this Location » Publication #164979
Title: SSR TRANSFERENCE AMONG ROSOIDEAE GENERA.

Author
item Lewers, Kimberly
item STAFNE, ERIC - UNIV. OF ARKANSAS
item CLARK, JOHN - UNIV. OF ARKANSAS
item WEBER, COURTNEY - CORNEL UNIV.
item GRAHAM, JULIE - SCOTTISH CR. RES. INST.

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/18/2004
Publication Date: 5/22/2004
Citation: Lewers, K.S., Stafne, E.T., Clark, J.R., Weber, C.A., Graham, J. 2004. Ssr transference among rosoideae genera.. Meeting Abstract.

Interpretive Summary:

Technical Abstract: SSR transference among Rosoideae genera Small fruit breeders working with Fragaria (strawberry) and Rubus species (raspberry and blackberry) are interested in molecular markers for marker-assisted selection. Simple Sequence Repeat molecular markers (SSRs) are desired for their many advantages. Several researchers working with SSR markers have reported that SSRs developed from one species can sometimes be used with other related species. If this is true among Fragaria and Rubus species, such SSR markers would be useful in transferring traits from one species to another, especially beneficial since it is not uncommon for a small fruit breeder to utilize more than one species. SSRs were derived from seven Rosoideae species representing Fragaria, Rubus, and Rosa, and tested with DNA from 16 strawberry, raspberry, and blackberry genotypes. Three mapping populations were used to study polymorphism detection: an interspecific strawberry population, and interspecific raspberry population, and a blackberry population. SSRs derived from any Fragaria species generally amplified a product from any other Fragaria species, and SSR polymorphism detection in the strawberry mapping population was about 71%. SSRs derived from Rubus species were less transferable across species. Of the red raspberry-derived SSRs, 74% amplified a product from NY322 red raspberry, 53% amplified a product from 'Jewel' black raspberry, and 32% amplified a product from two blackberry genotypes. Of the R. alcefolius SSRs, 25% amplified a product from bramble genotypes. About 10% of the Fragaria-derived SSRs will be useful for mapping in the two bramble populations, but none of the 14 Rubus tested with Fragaria genotypes amplified a product from any Fragaria genotype, and none of the 30 Rosa-derived SSRs amplified a product from any of the Fragaria or Rubus genotypes tested. In summary, SSRs for use with strawberry mapping and marker-assisted selection can be derived from any Fragaria species. However, because transference among Rubus species was not as successful, it may be necessary to develop raspberry-derived SSRs for use with raspberry and blackberry-derived SSRs for use with blackberry.