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ARS Home » Northeast Area » Boston, Massachusetts » Jean Mayer Human Nutrition Research Center On Aging » Research » Publications at this Location » Publication #165358
Title: EFFECT ON QUERCETIN INTAKE ON BIOMARKERS OF OXIDATIVE STRESS IN F344 RATS FED VITAMIN E REPLETE OR DEFICIENT DIETS

Author
item AMEHO, CLEMENT - TUFTS-HNRCA
item MARTIN, ANTONIO - TUFTS-HNRCA
item MILBURY, PAUL - TUFTS-HNRCA
item BLUMBERG, JEFFREY - TUFTS-HNRCA

Submitted to: Journal of Federation of American Societies for Experimental Biology
Publication Type: Abstract Only
Publication Acceptance Date: 2/1/2004
Publication Date: 3/23/2004
Citation: Ameho, C.K., Martin, A., Milbury, P.E., Blumberg, J. 2004. Effect on quercetin intake on biomarkers of oxidative stress in f344 rats fed vitamin e replete or deficient diets. Journal of Federation of American Societies for Experimental Biology. 18(5 Part II):A908.

Interpretive Summary:

Technical Abstract: Reports suggest consumption of flavonoid-rich foods may reduce the risk for chronic diseases in part through antioxidant mechanisms. Despite substantial data demonstrating antioxidant properties of flavonoids in vitro, little information is available supporting such an action in vivo. Therefore, we assessed the in vivo antioxidant potential of quercetin in weanling F344 rats fed diets replete (R, 30 mg a-tocopherol acetate/kg) or deficient (D) in vitamin E (vitE) for 12 wk. Rats were fed diets (n=13/group) as: (1) ad lib R-vitE; (2) pair-fed R-vitE; (3) R-vitE + 5.0 g quercetin/kg; (4) D-vitE; (5) D-vitE + 0.5 g quercetin/kg; (6) D-vitE + 5.0 g quercetin/kg. Plasma vitE was 18.0±0.6 µmol/L in R-vitE groups (1-3) and not detectable in D-vitE groups (4-6), (P<0.001). Serum pyruvate kinase was 1.3±0.06 and 10.7±0.37 U in the R-vitE and D-vitE groups, respectively (P<0.0083). Plasma protein carbonyls were 1.1±0.03 and 1.4±0.04 nmol/mg protein in the R-vitE and D-vitE groups, respectively (P<0.0083). Plasma malondialdehyde was 1.8+0.04 and 2.2+0.08mmol in the R-vitE and D-vitE groups, respectively (P<0.001). In this model quercetin interventions had no significant impact on any of these biomarkers of vitamin E status or oxidative stress.