Author
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Ow, David |
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Submitted to: In Vitro Cellular and Developmental Biology - Plants
Publication Type: Peer Reviewed Journal Publication Acceptance Date: 7/1/2004 Publication Date: 7/1/2005 Citation: Ow, D.W. 2005. Transgene management via multiple site-specific recombination systems. In Vitro Cellular and Developmental Biology - Plants 41(3):213-219(7). Interpretive Summary: This article describes a strategy on how site-specific recombination can be used to direct DNA precisely into the plant genome, remove unneeded DNA from the integration locus, transfer the transgenic locus to elite cultivars, and remove transgenes from pollen and/or seed. Technical Abstract: Current methods for creating transgenic varieties are labor and time intensive, comprised of the generation of hundreds of plants with random DNA insertions, screening for the few individuals with appropriate transgene expression and simple integration structure, and followed by a lengthy breeding process to introgress the engineered trait into cultivated varieties. Various modifications of existing methods have been proposed to speed up the different steps involved in plant transformation, as well as a few add-on technologies that seek to address issues related to biosafety or intellectual property. The problem with an assortment of independently developed improvements is that they do not integrate seamlessly into a single transformation system. This paper presents an integrated strategy for plant transformation, where the introduced DNA will be inserted precisely into the genome, the transgenic locus will be introgressed rapidly into field varieties, the extraneous transgenic DNA will be removed, the transgenic plants will be molecularly tagged, and the transgenic locus may be excised from pollen and/or seed. |
