Author
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Burri, Betty |
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Submitted to: Book Chapter
Publication Type: Book / Chapter Publication Acceptance Date: 1/7/2007 Publication Date: 3/31/2007 Citation: Burri, B.J. Analysis of vitamin e by hplc. Book Chapter. 2007. The Encyclopedia of Vitamin E. pp.172-181. Interpretive Summary: High performance liquid chromatography is the most common method for identifying and measuring vitamin E, an essential nutrient. This manuscript describes a common method, and reports on trends for new developments of this methodology. A variety of good high performance liquid chromatography methods are available for vitamin E analysis. Technical Abstract: HPLC (high performance liquid chromatography) is the major method for identifying and measuring vitamin E concentrations. A variety of good methods are available for vitamin E analysis. Reliable and sensitive methods have been developed for reversed-phase and normal phase columns, isocratic and gradient elutions, and fluorescent, electrochemical, and ultraviolet detectors. Satisfactory internal standards include tocol, tocopheryl acetate, and retinyl acetate. Current methods development focuses on analyzing vitamin E in difficult sample matrices, analyzing vitamin E metabolites, and most commonly on combining vitamin E analysis with the analysis of other micronutrients such as vitamin A and the carotenoids. |
