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Title: CHARACTERIZATION OF EXOPOLYSACCHARIDES PRODUCED FROM AUREOBASIDIUM PULLULANS

Author
item PRASONGSUK, S - CHULALONGKORN UNIV
item Leathers, Timothy
item KUHIRUN, M - CHULALONGKORN UNIV
item EVELEIGH, D - RUTGERS UNIV
item PUNNAPAYAK, H - CHULALONGKORN UNIV

Submitted to: Society of Industrial Microbiology Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 7/29/2004
Publication Date: 7/29/2004
Citation: Prasongsuk, S., Leathers, T.D., Kuhirun, M., Eveleigh, D.E., Punnapayak, H. 2004. Characterization of exopolysaccharides produced from Aureobasidium pullulans [abstract]. Society of Industrial Microbiology. p. 72.

Interpretive Summary:

Technical Abstract: Several black yeasts were isolated from habitats in Thailand, and all were positively identified as Aureobasidium pullulans var. pullulans. Their exopolysaccharides (EPSs) were produced by cultivating the yeast cells in liquid culture with various carbon and nitrogen sources. The maximal EPS yield was obtained from A. pullulans NRM2 (0.53 g.g**-1) using sucrose and peptone at day 7. The hexose content of the EPSs was in the range of 97-99%. Analysis with IR and NMR suggested that the EPSs were composed of pullulan, the spectra illustrating the alpha-configuration and with the anomeric carbon of alpha-1,4 and alpha-1,6 (1,4:1,6) in a 2:1 ratio. However, the degree of pullulanase sensitivity varied among the EPSs ranging from 56% to 97%. The molecular weights (and viscosities) of these EPSs decreased late in culture from the range of 547,000-2,270,000 Da on day 3 to 16,000-160,000 Da on day 7. Amylase activities increased up to 0.11 U/ml in the culture fluid during the 7-day period.