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Title: COMPLETE NUCLEOTIDE SEQUENCE AND GENOME ORGANIZATION OF GRAPEVINE LEAFROLL-ASSOCIATED VIRUS-3, TYPE MEMBER OF THE GENUS AMPELOVIRUS

Author
item Ling, Kai-Shu
item ZHU, HAI-YING - UNIVERSITY OF WASHINGTON
item Gonsalves, Dennis

Submitted to: Journal of General Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/4/2004
Publication Date: 6/5/2004
Citation: Ling, K., Zhu, H., Gonsalves, D. 2004. Complete nucleotide sequence and genome organization of grapevine leafroll-associated virus-3, type member of the genus Ampelovirus. Journal of General Virology. 85:2099-2102.

Interpretive Summary: This study represents the first complete genome sequence of a very widespread and important virus in grapevines. This information will help scientist develop control measures for this virus.

Technical Abstract: This study reports on the complete genome sequence of grapevine leafroll-associated virus-3, the type member of the genus Ampelovirus. The genome is 17 919 nt in size and contains 13 open reading frames (ORFs). Previously, the sequence of 13 154 nt of the 3 -terminal of the genome was reported. The newly sequenced portion contains a 158 nt 5 UTR, a single papain-like protease and a methyltransferase-like domain (MT). ORF1a encodes a large polypeptide with a molecular mass of 245 kDa. With a predicted +1 frameshift, the large fusion protein generated from ORF1a/1b would produce a 306 kDa polypeptide. Phylogenetic analysis using MT domains further supports the creation of the genus Ampelovirus for mealy-bug-transmitted viruses in the family Closteroviridae.