Skip to main content
ARS Home » Research » Publications at this Location » Publication #166227
Title: TRANSFORMATION OF TARO (COLOCASIA ESCULENTA) WITH A RICE CHITINASE GENE

Author
item HE, XIAOLING - UNIVERSITY OF HAWAII
item MIYASAKA, SUSAN - UNIVERSITY OF HAWAII
item Fitch, Maureen
item ZHU, Y. - HI AG RES CNT
item Moore, Paul

Submitted to: In Vitro Cellular and Developmental Biology - Plants
Publication Type: Abstract Only
Publication Acceptance Date: 3/1/2004
Publication Date: 5/22/2004
Citation: He, X., Miyasaka, S., Fitch, M.M., Zhu, Y.J., Moore, P.H. 2004. Transformation of taro (Colocasia esculenta) with a rice chitinase gene. In Vitro Cell and Development Biology. 40:68-A. Abstract p-2104. 2004.

Interpretive Summary:

Technical Abstract: To obtain partial resistance to a major taro fungal disease, a rice chitinase gene (CHI11) in a plasmid vector (pBI121) was introduced into taro calli using high-velocity particle bombardment. Highly regenerative taro calli of cv. Bun Long were chopped into small segments and used as targets of transformation. Transformed calli were selected on Murashige and Skoog (MS) medium containing 2 mg L-1 BA, 1 mg L-1 NAA and 50 mg L-1 geneticin (G418). The candidate transformant calli were screened by polymerase chain reaction (PCR) amplification using a primer for the CHI11 gene and two positive lines were obtained. Shoots were induced from these two positive lines, and screened by PCR amplification again. Only one positive line was obtained. The transformant was further confirmed by Southern blot analysis. This transgenic plant line is being further propagated for fungal disease resistance assays.