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Title: GLOBAL BYDV/CYDV SEQUENCING PROJECT

Author
item Gray, Stewart
item MILLER, W - IOWA STATE UNIVERSITY
item Anderson, Joseph
item GAI, X - IOWA STATE UNIVERSITY

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 3/30/2004
Publication Date: 6/30/2004
Citation: Gray, S.M., Miller, W.A., Anderson, J.M., Gai, X. 2004. Global bydv/cydv sequencing project. Phytopathology. 94:S71.

Interpretive Summary:

Technical Abstract: The yellow dwarf viruses (YDVs), Barley yellow dwarf (BYDV) and Cereal yellow dwarf (CYDV), are, world wide, the most economically important viruses of wheat, barley and oats. Resistance genes are limited and many YDV isolates break resistance. YDVs have remarkable genetic and biological diversity. The handful of isolates that have been sequenced have resulted in the original BYDV being reclassified into at least three virus species in two different genera. Plant breeders and pathologists around the world attempting to breed resistant crops have discovered that YDV isolates within the same serotype can cause very different symptoms and respond differently to host resistance genes. This project will determine complete nucleotide sequences of up to 100 biologically important YDV isolates from around the world. We present methods for rapid full-length RT-PCR, sequencing, and construction and testing of full-length infectious clones. A large database of sequence information, detection methods and biological phenotypes will be posted on a web site to serve as a resource for epidemiological and molecular mechanistic studies. This will be useful for mapping vector specificity determinants, predicting key RNA secondary structures, and determining differential response to host resistance genes. We invite new collaborators to contribute isolates for sequencing. Funds are also available to train researchers in sequencing and molecular detection methods to facilitate their resistance and epidemiological studies.