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ARS Home » Southeast Area » Charleston, South Carolina » Vegetable Research » Research » Publications at this Location » Publication #166531
Title: PROTOCOL FOR DNA EXTRACTION FROM WATERMELON LEAVES FOR SSR MARKER STUDIES

Author
item GUSMINI, GABRIELE - NCSU
item WEHNER, TODD - NCSU
item JOOBEUR, TAREK - NCSU
item DEAN, RALPH - NCSU
item Levi, Amnon

Submitted to: Cucurbit Genetics Cooperative Report
Publication Type: Abstract Only
Publication Acceptance Date: 8/15/2004
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Due to the high amounts of phenolic compounds in watermelon leaves, it is difficult to isolate high quality DNA from their tissue employing isolation procedures commonly used with various plant species. In this study, a procedure for isolating high-quality DNA from a few watermelon leaf samples was modified for isolating DNA from a large number of leaf samples (24 samples per run; 500 mg of ground fresh leaf tissue). The modified DNA isolation procedure is suitable for fast analysis of watermelon genome using simple sequence repeat (SSR), randomly amplified polymorphic DNA (RAPD), or sequenced characterized amplified regions (SCAR) markers.