Skip to main content
ARS Home » Research » Publications at this Location » Publication #167557
Title: PHOTOAUTOTROPHIC MEDIUM AND PPM(TM) HELP TO ALLEVIATE LOSSES FROM BACTERIAL CONTAMINATION IN PAPAYA MICROPROPAGATION

Author
item Fitch, Maureen
item LEONG, T. - HARC
item SAITO, N. - HARC
item YAMAMOTO, G. - HARC
item DELA CRUZ, A. - HARC
item YEH, A. - HARC
item WHITE, S. - HARC
item Maeda, Sharyn
item Moore, Paul

Submitted to: In Vitro Cellular and Developmental Biology - Plants
Publication Type: Abstract Only
Publication Acceptance Date: 2/15/2004
Publication Date: 5/1/2004
Citation: Fitch, M.M., Leong, T., Saito, N., Yamamoto, G., Dela Cruz, A., Yeh, A., White, S., Maeda, S.H., Moore, P.H. 2004. Photoautotrophic medium and ppm(tm) help to alleviate losses from bacterial contamination in papaya micropropagation. In Vitro Cell Dev Biol (2004) 40:32A.

Interpretive Summary: Abstract only.

Technical Abstract: Contamination problems, apparently with bacteria, were encountered in micropropagation of papaya. Although axenic cultures were re-isolated, the occasional re-appearance of contaminants provided reason for us to devise a method in which losses due to contamination could be minimized. Two modifications resulted in improved plant survival and growth. Rooted shoots were grown in photoautotrophic rooting medium devoid of sucrose since apparently contaminated shoots had turned yellow and grown poorly in medium containing sucrose. Rooted shoots in the photoautotrophic medium remained green and healthy in appearance, formed numerous roots, and their ex vitro survival rate was close to 90%.