A DIAGNOSTIC AID FOR DIFFERENTIATING INFECTED FROM VACCINATED POULTRY BASED ON ANTIBODIES TO THE NONSTRUCTURAL (NS1) PROTEIN OF INFLUENZA A VIRUS

Authors: TUMPEY, TERRENCE - CDC - ATLANTA, GA; ALVAREZ, RENE - CDC - ATLANTA, GA; Swayne, David; Suarez, David

Submitted to: Journal of Clinical Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/27/2004
Publication Date: 2/10/2005
Citation: Tumpey, T., Alvarez, R., Swayne, D.E., Suarez, D.L. 2005. A diagnostic aid for differentiating infected from vaccinated poultry based on antibodies to the nonstructural (ns1) protein of influenza a virus. Journal of Clinical Microbiology. 43:676-683.

Interpretive Summary: Killed avian influenza virus vaccines are used in some parts of the world to protect birds from avian influenza. Serological tests to monitor success of vaccination cannot distinguish between vaccinated birds and birds infected with the live virus. A new ELISA test was developed that detected antibodies against NS1 protein of avian influenza. Vaccinated birds were negative for NS1 antibodies but virus-infected birds had antibodies to the NS1 protein. This new test can be used to differentiated infected from vaccinated animals (DIVA).

Technical Abstract: Not required.