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ARS Home » Pacific West Area » Corvallis, Oregon » National Clonal Germplasm Repository » Research » Publications at this Location » Publication #167936
Title: CRYOPRESERVATION BY VITRIFICATION

Author
item Reed, Barbara

Submitted to: World Wide Web Corvallis ARS GRIN Home Page
Publication Type: Other
Publication Acceptance Date: 7/7/2004
Publication Date: 7/7/2004
Citation: Reed, B.M. 2004. Cryopreservation by vitrification [educational web presentation]. http://www.ars-grin.gov/cor/presentations/PVS2Cryo_files/frame.htm

Interpretive Summary: Cryogenic storage of apical meristems is being used by the US Department of Agriculture, Agricultural Research Service, National Plant Germplasm System as base (long-term backup) storage for several temperate fruit (pear, currant, gooseberry, blackberry, raspberry) and specialty crops (hops, mint, grass). Cryopreservation protocols are defined for many types of plants and many plant forms (seeds, pollen, dormant buds, and apical meristems). Long-term storage for vegetatively propagated plants has always been difficult. Cryogenic storage provides security for the select cultivars important to horticulture and agriculture. Several protocols are now available for use with diverse crops. At the National Clonal Germplasm Repository vitrification with PVS2 is a successful technique for some crop types. This web presentation outlines the steps for the basic technique. With proper manipulation most clonally propagated plants can be stored for future generations.

Technical Abstract: Cryogenic storage of apical meristems is being used by the US Department of Agriculture, Agricultural Research Service, National Plant Germplasm System as base (long-term backup) storage for several temperate fruit (pear, currant, gooseberry, blackberry, raspberry) and specialty crops (hops, mint, grass). Cryopreservation protocols are defined for many types of plants and many plant forms (seeds, pollen, dormant buds, and apical meristems). Long-term storage for vegetatively propagated plants has always been difficult. Cryogenic storage provides security for the select cultivars important to horticulture and agriculture. Several protocols are now available for use with diverse crops. At the National Clonal Germplasm Repository vitrification with PVS2 is a successful technique for many crop types. This web presentation outlines the steps for the basic technique. With proper manipulation most clonally propagated plants can be stored for future generations.