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ARS Home » Plains Area » Clay Center, Nebraska » U.S. Meat Animal Research Center » Genetics and Animal Breeding » Research » Publications at this Location » Publication #169144
Title: DEVELOPMENT AND TESTING OF A HIGH-DENSITY CDNA MICROARRAY RESOURCE FOR CATTLE

Author
item SUCHYTA, STEVEN - MICHIGAN STATE UNIV
item SIPKOVSKY, SUE - MICHIGAN STATE UNIV
item KRUSKA, RACHAEL - MICHIGAN STATE UNIV
item JEFFERS, ABRA - MICHIGAN STATE UNIV
item MCNULTY, AMANDA - MICHIGAN STATE UNIV
item COUSSENS, MATTHEW - MICHIGAN STATE UNIV
item TEMPELMAN, ROBERT - MICHIGAN STATE UNIV
item HALGREN, ROBERT - MICHIGAN STATE UNIV
item SAAMA, PETER - MICHIGAN STATE UNIV
item BAUMAN, DALE - CORNELL UNIV, NY
item BOISCLAIR, YVES - CORNELL UNIV, NY
item BURTON, JEANNE - MICHIGAN STATE UNIV
item COLLIER, ROBERT - UNIV ARIZONA, TUCSON
item DEPETERS, EDWARD - UNIV CALIF, DAVIS
item FERRIS, THEODORE - MICHIGAN STATE UNIV
item LUCY, MATTHEW - UNIV MO, COLUMBIA
item MCGUIRE, MARK - UNIV IDAHO, MOSCOW
item MEDRANO, JUAN - UNIV CALIF, DAVIS
item OVERTON, THOMAS - CORNELL UNIV, NY
item Smith, Timothy - Tim
item SMITH, GEORGE - MICHIGAN STATE UNIV
item SONSTEGARD, TAD - 1265-90-00
item SPAIN, JAMES - UNIV MO, COLUMBIA
item SPIERS, DONALD - UNIV CALIF, DAVIS
item JIANBO, YAO - MICHIGAN STATE UNIV
item COUSSENS, PAUL - MICHIGAN STATE UNIV

Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 1/10/2004
Publication Date: 1/10/2004
Citation: Suchyta, S.P., Sipkovsky, S., Kruska, R., Jeffers, A., Mcnulty, A., Coussens, M.J., Tempelman, R.J., Halgren, R.A., Saama, P.M., Bauman, D.E., Boisclair, Y.R., Burton, J.L., Collier, R.J., Depeters, E.J., Ferris, T.A., Lucy, M.C., Mcguire, M.A., Medrano, J.F., Overton, T.4., Smith, T.P., Smith, G.W., Sonstegard, T.S., Spain, J.N., Spiers, D.E., Jianbo, Y., Coussens, P.M. 2004. Development and testing of a high-density cDNA microarray resource for cattle. Proc., Plant and Animal Genome Abstracts. P831. p. 278.

Interpretive Summary:

Technical Abstract: A cDNA microarray resource has been developed with the goal of providing integrated functional genomics resources for cattle. The National Bovine Functional Genomics Consortiums's (NBFGC) EST collection was established in 2001 to develop resources for functional genomics research. The NBFGC EST collection and microarray contains 18,263 unique transcripts, derived from many different tissue types and various physiologically important states within these tissues. The NBFGC microarry has been tested for false positive rates using self-hybridizations and was shown to yield robust results in test microarray experiments. A web-accessible database has been established to provide pertinent data related to NBFGC clones, including sequence data, BLAST results, and ontology information. The NBFGC microarray represents the largest cDNA microarray for a livestock species prepared to date and should prove to be a valuable tool in studying genome-wide gene expression in cattle.