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ARS Home » Pacific West Area » Corvallis, Oregon » National Clonal Germplasm Repository » Research » Publications at this Location » Publication #169443
Title: IN VITRO COLD STORAGE: A RELIABLE METHOD OF STONE FRUIT GERMPLASM PRESERVATION

Author
item KOVALCHUK, I. - KAZAKH INSTITUTE
item Reed, Barbara

Submitted to: Research Collection of Agricultural Academy, Almaty
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/20/2004
Publication Date: 7/20/2004
Citation: Kovalchuk, I.Y., Reed, B.M. 2004. In vitro cold storage: a reliable method of stone fruit germplasm preservation. In: Proc. Intern. Sci. Conf. The Strategy of Scientific Ensuring of Horticulture: Reality and Perspectives. Almaty Agricultural University. p. 136-140. (in Russian)

Interpretive Summary: Apple, raspberry, blackberry, cherry and plum in-vitro stored plants were tested for the ability to withstand refrigerated storage. The microplants in test tubes or plastic storage bags were placed in the chamber with low positive temperature (5 '/ 45 F),low light intensity (7 'mol m-2s-1) and a 10 hour photoperiod. Several types of growth regulators and nutrient combinations were tested. There were variations among the cultivars, but the majority of strawberries and raspberries have remained alive for up to 1 and a half years, and the cherry began to die after half of a year. Storage in plastic tissue culture bags was more successful than that in glass tubes.

Technical Abstract: Apple, raspberry, blackberry, cherry and plum in-vitro stored plants were tested for the ability to withstand refrigerated storage. The microplants in test tubes or plastic storage bags were placed in the growth chamber with low temperature (5 '/ 45 F),low light intensity (7 'mol m-2s-1) and a 10 hour photoperiod. Several types of growth regulators and nutrient combinations were tested as well as vessel types. The obsevations showed that the reaction of species and cultivars to in vitro cold long-term storage is not identical. So the plant condition of wild strawberry and raspberry was better, than for plum, cherry and sweet cherry. The majority of strawberry and raspberry genotypes remained viable for 15-18 months, and the cherry began to die in 6-9 months of the storage. Influence of the different contents of growth regulators, carbohydrates and volume of vessels in which the plants were stored was tested. The basic growth medium was MS with 6-benzylaminopurine (BAP), indole-3-butyric acid (IBA), abscisic acid (ABA) were used as the growth regulators. The carbohydrates sucrose and mannitol were also tested. Probably low level of the storage viability is explained by limited gas ex-change which taking place in the glass tubes and vessels, therefore now all genotypes were transferred for storage in plastic, air-penetrable packages. The results improved greatly in this type of storage container.