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Title: LC/MS ANALYSIS OF BOA AND MBOA AND THEIR PRECURSORS IN RYE

Author
item Rice, Clifford
item ADAM, FREDERICK - UNIV. OF MARYLAND
item Abdul Baki, Aref
item Park, Yong
item Teasdale, John

Submitted to: Agronomy Society of America, Crop Science Society of America, Soil Science Society of America Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 8/11/2004
Publication Date: 11/3/2004
Citation: Rice, C., Adam, F.M., Abdul-Baki, A.A., Park, Y.I., Teasdale, J.R. 2004. LC/MS analysis of BOA and MBOA and their precursors in rye. Proc. Amer. Soc. Agronomy, Div.5-8, p.371 (Poster No. 1956).

Interpretive Summary:

Technical Abstract: A new LC/MS-MS method for analysis of allelopathic chemicals in Rye was developed. Methoxy-substituted forms of the hydroxamic group were re-discovered along with measuring the main hydroxamic moieties, DIBOA and BOA, and small amounts of their precursors, the glucosylated forms. Samples were also examined for Beta-phenylactic acid (PLA) and Beta-hydroxybutyric acid (HBA), and small amounts were measured. The preparation method involved aqueous incubation of the dried/ground plant materials (1:15 wt/wt, plant:water) for 12 hours which dictated that a greater quantity of the aglucones would be present since the plants have natural levels of Beta-glucosidases that degraded the glucosylated forms. The analytical method is reproducible, is sensitive, and requires no sample cleanup and very simple preparation procedures. MDLs were as follows: hydroxybutyric - 15 ng/g, phenylacetic - 3.4 ng/g, DIBOA-glucose - 2.8 ng/g, DIBOA - 1.6 ng/g, BOA - 3.0 ng/g, DIMBOA-glucose - 10.5 ng/g, DIMBOA - 0.3 ng/g, MBOA - 2.13 ng/g.