Skip to main content
ARS Home » Midwest Area » St. Paul, Minnesota » Plant Science Research » Research » Publications at this Location » Publication #170505
Title: STUDYING REPETITIVE SEQUENCES USING OAT-MAIZE RADIATION HYBRID LINES

Author
item OKAGAKI, RON - UNIVERSITY OF MINNESOTA
item KYNAST, RALF - UNIVERSITY OF MINNESOTA
item STEC, ADRIAN - UNIVERSITY OF MINNESOTA
item SCHMIDT, CHRISTINA - UNIVERSITY OF MINNESOTA
item JACOBS, MORRISON - UNIVERSITY OF MINNESOTA
item ODLAND, WADE - UNIVERSITY OF MINNESOTA
item CABRAL, CANDIDA - UNIVERSITY OF MINNESOTA
item WALCH, MATT - UNIVERSITY OF MINNESOTA
item GALATOWITSCH, MARK - UNIVERSITY OF MINNESOTA
item HUETTL, PAUL - UNIVERSITY OF MINNESOTA
item SURESH, JAYANTI - UNIVERSITY OF MINNESOTA
item DOSDALL, ANDREW - UNIVERSITY OF MINNESOTA
item NINKOVIC, JANA - UNIVERSITY OF MINNESOTA
item GILLEN, JENN - UNIVERSITY OF MINNESOTO
item YOON, CLAIRE - UNIVERSITY OF MINNESOTA
item RETZEL, ERNIE - UNIVERSITY OF MINNESOTA
item Rines, Howard
item PHILLIPS, RONALD - UNIVERSITY OF MINNESOTA

Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 10/10/2004
Publication Date: 1/15/2005
Citation: Okagaki, R., Kynast, R., Stec, A., Schmidt, C., Jacobs, M., Odland, W., Cabral, C., Walch, M., Galatowitsch, M., Huettl, P., Suresh, J., Dosdall, A., Ninkovic, J., Gillen, J., Yoon, C., Retzel, E., Rines, H.W., Phillips, R. 2005. Studying repetitive sequences using oat-maize radiation hybrid lines [abstract]. Plant and Animal Genome XIII Conference Abstracts. p. 171.

Interpretive Summary:

Technical Abstract: The oat-maize radiation hybrid (RH) mapping project is developing RH lines from chromosome addition lines (oat plants that carry a single maize chromosome). Physical maps produced will complement existing genetic maps. In addition to their expected uses in physical mapping of genes and BAC-end sequences, we have found that RH lines are useful for studying repetitive sequences; two such uses are described here. First, RH lines provide a means for rapidly surveying the distribution along a chromosome of individual families of repetitive elements. PCR assays of RH lines that each contain a single well-defined segment of a maize chromosome can reveal which region(s) of a maize chromosome contains members of that family of repetitive elements. Second, RH lines that separate a maize chromosome into segments provide a means to study the concentration of repetitive versus low-copy sequences along a chromosome. Gene rich and gene poor areas of a chromosome may be identified. In addition, we will present a summary of new oat-maize chromosome addition lines from maize B73 and other important inbred lines and RH mapping panels for maize chromosomes 1, 2, 4, 6, and 9. This material is based upon work supported by the National Science Foundation under Award No. 0110134.