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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Ruminant Diseases and Immunology Research » Research » Publications at this Location » Publication #170863
Title: DETECTION OF THE CYTOKINE IL-8 USING LASER-INDUCED CAPILLARY ELECTROPHORESIS

Author
item Kimura, Kayoko
item Schmerr, Mary Jo

Submitted to: High Performance Capillary Electrophoresis
Publication Type: Abstract Only
Publication Acceptance Date: 10/15/2004
Publication Date: 2/13/2005
Citation: Kimura, K., Schmerr, M. 2005. Detection of the cytokine IL-8 using laser-induced capillary electrophoresis [abstract]. High Performance Capillary Electrophoresis Meeting.

Interpretive Summary:

Technical Abstract: The cytokine IL-8 plays an important role in the inflammatory response. Assays which measure the cytokines at physiological levels in the low pg/ml range are not available. In this study, we explored the use of capillary electrophoresis using laser-induced fluorescence (CE-LIF) and FITC-labeled protein A as a method to measure immunocomplexes formed from specific antibody and human recombinant IL-8. After mixing these three components and incubating overnight at 20 C, the sample was analyzed by CE-LIF. The detection limit of the assay was less than 1.0 pg/ml. The amount of bovine IL-8 that can be detected in ELISA is 50 pg/ml. When IL-8 was measured in plasma samples from cows with physiological problems related to parturition, a good correlation with the ELISA results were found with this assay. The amount of IL-8 in some of the samples was below the detection limits of the ELISA.