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Title: BMIGI: A DATABASE OF CDNAS EXPRESSED IN BOOPHILUS MICROPLUS, THE TROPICAL/SOUTHERN CATTLE TICK

Author
item Guerrero, Felicito
item Miller, Robert
item ROUSSEAU, M-E - INIMEX PHAR. VANCOUVER BC
item SUNKARA, S - INIMEX PHAR. VANCOUVER BC
item QUACKENBUSH, J - TIGR, ROCKVILLE MD
item LEE, Y - TIGR, ROCKVILLE MD
item NENE, V - TIGR, ROCKVILLE MD

Submitted to: Insect Biochemistry and Molecular Biology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/26/2004
Publication Date: 6/1/2005
Citation: Guerrero, F.D., Miller, R.J., Rousseau, M.-E, Sunkara, S., Quackenbush, J., Lee, Y., Nene, V. 2005. BmiGI: A database of cDNAs expressed in Boophilus microplus, the tropical/southern cattle tick. Insect Biochemistry and Molecular Biology. 35:585-595.

Interpretive Summary: We have synthesized a Boophilus microplus DNA library using tick larvae which had been subjected to different treatments, including acaricide exposure, heat shock, desiccation, cold shock, host odor, and infection with Babesia bovis. For the acaricide exposure experiments, we used several strains of ticks which varied in their levels of susceptibility to different classes of acaricide, including pyrethroid, organophosphate and amitraz. We also included RNA purified from samples of different developmental stages and tick organs. DNA was prepared from 11,520 clones from the library and both 5' and 3' sequence data was generated from each clone. The sequence data was used to search public sequence databases and a B. microplus gene index was constructed, consisting of 8,270 unique sequences and their associated putative functional assignments, when available, which can be viewed at the TIGR website (http://www.tigr.org/tdb/tgi). A number of new sequences were identified which might be involved in regulating the tick's resistance to acaricides.

Technical Abstract: We used an expressed sequence tag approach to study the genome of the southern cattle tick, Boophilus microplus. A normalized cDNA library was synthesized from pooled RNA purified from tick larvae which had been subjected to different treatments, including acaricide exposure, heat shock, desiccation, cold shock, host odor, and infection with Babesia bovis. For the acaricide exposure experiments, we used several strains of ticks which varied in their levels of susceptibility to pyrethroid, organophosphate and amitraz. We also included RNA purified from samples of different developmental stages and tick organs. Template was prepared from 11,520 clones and both 5' and 3' EST data was generated from each clone. The sequence data was used to search public protein databases and a B. microplus gene index was constructed, consisting of 8,270 unique sequences and their associated putative functional assignments, when available, which can be viewed at the TIGR website (http://www.tigr.org/tdb/tgi). A number of ESTs were identified which possessed significant sequence similarity to genes which might be involved in resistance to acaricides.