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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Cereal Crops Research » Research » Publications at this Location » Publication #171643
Title: COMPARATIVE ANALYSES OF A GENE RICH REGION OF WHEAT CHROMOSOME 5B WITH RICE USING EXPRESSED SEQUENCES.

Author
item LU, HUANGJUN - PLNT SCI, NDSU, FARGO ND
item Faris, Justin

Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 10/3/2004
Publication Date: 1/12/2005
Citation: Lu, H., Faris, J.D. Comparative analyses of a gene rich region of wheat chromosome 5b with rice using expressed sequences.. Plant and Animal Genome XIII Abstracts p. 259.

Interpretive Summary:

Technical Abstract: EST and genome sequencing data have provided the basis for comparative genomics between wheat and rice. In this study, markers representing expressed sequences within the wheat deletion interval 5BL 0.75-0.76 were used to determine the level of colinearity of this genomic region with rice. A population consisting of 117 recombinant substitution lines (RSLs) derived from a cross of Chinese Spring (CS) x CS Triticum dicoccoides chromosome 5B substitution line (CS-Dic5B) was used to develop a genetic map corresponding to the deletion interval. We mapped 35 expressed sequence markers as RFLPs or SSCPs, which resulted in a map of 51.4 cM in length. Of these markers, 19 (54%) detected homologous sequences in rice genome. Analyses of colinearity using expressed sequences that detected rice homologs indicated a lack of conservation. Small regions of colinearity with rice chromosomes 3 and 9 were found, but multiple breaks, interruptions, and rearrangments exist. These anomalies will make it extremely difficult to use rice as a model for positional cloning of wheat genes in this region due to the lack of conservation.