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Title: MELEAGRIS GALLOPAVO LEPTIN RECEPTOR GENE-RELATED PROTEIN (LEPR-GRP) MRNA, COMPLETE CDS

Author
item Richards, Mark
item Poch, Stephen

Submitted to: Genbank
Publication Type: Abstract Only
Publication Acceptance Date: 12/1/2004
Publication Date: 12/6/2004
Citation: Richards, M.P., Poch, S.M. 2004. Meleagris gallopavo leptin receptor gene-related protein (LEPR-GRP) MRNA, complete CDS [abstract]. GenBank. Acession No. AY826977.

Interpretive Summary:

Technical Abstract: A portion of the leptin receptor gene-related protein (LEPR-GRP) gene was isolated, sequenced and characterized from turkey (Meleagris gallopavo) tissue using primer-directed reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of 5' and 3' cDNA ends (5' and 3' RACE). Direct sequencing of overlapping PCR-generated DNA fragments identified and confirmed a total of 1466 contiguous bases corresponding to the LEPR-GRP mRNA (cDNA) including the complete coding region, and portions of the 5'- and 3'-untranslated regions. From the nucleotide sequence, a protein consisting of 131 amino acids was predicted that showed identities of 100%, 87%, and 87% with corresponding LEPR-GRP amino acid sequence data for chicken, human, and mouse, respectively. The predicted amino acid sequence demonstrated distinct features including: 1) three putative trans-membrane domains and 2) a putative JAK2 binding motif (Box 1). Further, the predicted protein amino acid sequence indicated a high degree of homology with a yeast protein, vacuolar protein sorting 55 (Vps55). Both Vps55 and leptin receptor gene-related protein are reported to be important for functional membrane trafficking to the vacuole/lysosome of eukaryotic cells. This sequence data is the first demonstration of a LEP-GRP gene in turkeys. Such data will be useful in studying the structure and expression of avian LEP-GRP genes, as well as, in determining the function of this protein in poultry.