Author
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Klasson, Kjell |
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Submitted to: Meeting Abstract
Publication Type: Abstract Only Publication Acceptance Date: 1/6/2005 Publication Date: 5/1/2005 Citation: Klasson, K.T. 2005. Spreadsheet tools for determination of derivatives and microbial kinetics (abstract). 27th Symposium on Biotechnology for Fuels and Chemicals. Paper No. 6-55. Interpretive Summary: Technical Abstract: Determination of enzyme kinetics, microbial growth rates, substrate utilization rates, and product accumulation rates requires derivatives to be calculated with a method that can be duplicated and yield consistent results. Here I discuss two methods that have been suggested in the literature and provide spreadsheet formulas for the needed equations. A cubic spline method proved more versatile in its use even though the spreadsheet programming was elaborate. The methods discussed were tested with published literature data for cell growth and a preferred technique was developed based on smoothing the data and using this smooth data for rate calculations. |
