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Title: NEONATAL LAMB RESPIRATORY TRACT DENDRITIC CELLS AND THE EFFECTS OF RSV INFECTION

Author
item FACH, SASHA - IOWA STATE UNIVERSITY
item MEYERHOLZ, DAVID - IOWA STATE UNIVERSITY
item GALLUP, JACK - IOWA STATE UNIVERSITY
item ACKERMANN, MARK - IOWA STATE UNIVERSITY
item Lehmkuhl, Howard
item Sacco, Randy

Submitted to: Federation of American Societies for Experimental Biology Conference
Publication Type: Abstract Only
Publication Acceptance Date: 12/15/2004
Publication Date: 4/2/2005
Citation: Fach, S.J., Meyerholz, D.K., Gallup, J.M., Ackermann, M.R., Lehmkuhl, H.D., Sacco, R.E. 2005. Neonatal lamb respiratory tract dendritic cells and the effects of RSV infection [abstract]. Federation of American Societies for Experimental Biology Conference. 19:A403.

Interpretive Summary:

Technical Abstract: Neonatal children and animals are highly susceptible to respiratory syncytial virus (RSV) infection, the most prominent causative agent of respiratory disease worldwide. Little information is available describing how RSV affects respiratory tract dendritic cells (rtDC) in vivo, especially in a naturally susceptible host. The objective of this study was to determine the effects of RSV infection in neonatal lambs on rtDCs by phenotypic and functional assays. Neonatal lambs were inoculated for three or five days with a bovine strain of RSV (BRSV) or control media and the lungs were harvested. After enzymatic digestion, isolation of rtDCs was obtained by magnetic sorting on CD11c+ cells. Characterization of rtDCs was determined by EM, flow cytometry and tracer endocytosis. Ultrastructural analysis showed characteristic dendrites on the sorted rtDCs. The mean fluorescence intensity of MHC class I, CD86 and CD14 on rtDCs from infected animals was increased over controls but MHC class II was similar. Tracer endocytosis analysis showed minimal uptake via mannose receptors but the rtDCs were still capable of macropinocytosis. BRSV was detected by IHC staining on frozen lung sections, and by PCR and virus reisolation on the sorted rtDCs. Based on these observations rtDCs contain BRSV and display a mature phenotype.