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Submitted to: Plant Methods
Publication Type: Peer Reviewed Journal Publication Acceptance Date: 9/7/2009 Publication Date: 10/31/2009 Citation: Slovin, J.P. 2009. An inbred line of the diploid strawberry Fragaria vesca f. semperflorens for genomic and molecular genetic studies in the Rosaceae. Plant Methods. 5:15. Interpretive Summary: The commercially grown strawberry, Fragaria ananassa, has eight copies of each chromosome and is difficult to do genetic studies with. The small woodland strawberry, Fragaria vesca, has only two of each type of chromosome and is more amenable to laboratory studies. We have developed a line of F. vesca into a useful tool for testing the function of genes from all kinds of strawberries, and for doing the genetic analyses required to support the findings. This line, called 5AF7, was developed by inbreeding so that most of the genes on one member of any pair of chromosomes are the same as on the other member of the pair. This documented inbred line will facilitate comparisons between experiments where quantitative assessments of a trait need to be made. 5AF7 may also prove useful for testing genes from other related crops such as apple and peach. This line will be used by scientists studying genes in strawberry and other plants in the Rose family. Technical Abstract: An inbred line, 5AF7, of the diploid strawberry Fragaria vesca f. semperflorens var. Yellow Wonder was developed for testing the functions of genes in strawberry. These dayneutral plants have a small genome, a short life cycle of 3.5 - 4 months, and will flower and produce fruit in 4-inch pots in a growth chamber. 5AF7 is very similar in appearance to its progenitor, is runnerless, forms abundant branch crowns, and produces highly aromatic yellow fruit throughout the year in the greenhouse. F. vesca can be transformed with Agrobacterium tumefaciens, making these plants suitable for gene knockout and overexpression studies. |
