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Title: STRUCTURALLY INTEGRATED ORGANIC LIGHT-EMITTING DEVICE-BASED SENSORS FOR OXYGEN, GLUCOSE, HYDRAZINE, AND ANTHRAX

Author
item SHINAR, RUTH - IOWA STATE UNIVERSITY
item CHOUDHURY, BHASKAR - IOWA STATE UNIVERSITY
item ZH0U, ZHAOQUN - IOWA STATE UNIVERSITY
item WU, HAI-SHENG - ISU AND MINN STATE U
item TABATABAI, LOUISA
item SHINAR, JOSEPH - IOWA STATE UNIVERSITY

Submitted to: International Society for Optical Engineering
Publication Type: Proceedings
Publication Acceptance Date: 10/25/2004
Publication Date: 12/1/2004
Citation: Shinar, R., Choudhury, B., Zh0u, Z., Wu, H., Tabatabai, L.B., Shinar, J. 2004. Structurally integrated organic light-emitting device-based sensors for oxygen, glucose, hydrazine, and anthrax. International Society for Optical Engineering. Vol. 5588:59-69,2004.

Interpretive Summary: An organic light emitting device (OLED) was constructed to detect and measure luminescent chemical and biological reactions. The application of this device was demonstrated for measuring oxygen, glucose, hydrazine and anthrax lethal factor (LF). The glucose and oxygen sensors were constructed for measuring glucose or oxygen contents of biological samples and is based on the reaction between glucose and oxygen catalyzed by glucose oxidase. The hydrazine sensor was constructed for measuring environmental contamination by hydrazine, a highly toxic, but valuable propellant for space shuttles, and a common precursor in the synthesis of some polymers, plasticisers and pesticides. The hydrazine sensor is based on the reaction between non-luminescent anthracene-2,3-dicarboxaldehyde and hydrazine or hydrazine sulfate, which generates a luminescent product. The anthrax sensor was constructed to measure the presence of anthrax lethal factor expressed by Bacillus anthracis. The anthrax sensor is based on the detection of a luminescent cleavage product after LF action on a synthetic peptide substrate labeled with a fluorescence resonance energy transfer (FRET, donor-acceptor) pair.

Technical Abstract: Application of the new platform for luminescent chemical and biological sensors, i.e., the structural integration of an OLED excitation source and the sensing element, is described for the detection of oxygen, glucose, hydrazine, and anthrax. The oxygen and glucose sensors are operable in either the photoluminescence (PL) intensity (IPL) mode or the PL lifetime ('PL) mode, where the change in IPL or 'PL, respectively, of an oxygen-sensitive dye, yields the oxygen level. In the 'PL mode, the need for frequent sensor calibration, which remains a challenge in real-world sensing applications, is eliminated. For the measurement of the oxygen level, in either gaseous or aqueous media, and glucose concentration, the performance of sensors based on [4,4'-bis(2,2'-diphenylvinyl)-1,1'-biphenyl (DPVBi) OLEDs]/[tris(4,7-diphenyl-1,10-phenanthroline) Ru (II) (Ru(dpp) dye] are compared to those of [tris(8-hydroxy quinoline) Al (Alq3) OLEDs]/[Pt octaethyl porphyrin (PtOEP) dye]. OLED-based hydrazine and anthrax sensors are also described. The hydrazine sensor is based on the reaction between nonluminescent anthracene-2,3-dicarboxaldehyde (ADA) and hydrazine or hydrazine sulfate, which generates a luminescent product. The anthrax sensor is based on the cleavage of certain peptides by anthrax-secreted lethal factor (LF) enzyme. As the LF cleaves a Förster resonance energy transfer (FRET) donor-acceptor pair-labeled peptide, and the two cleaved segments are separated, the PL of the donor, previously absorbed by the acceptor, becomes detectable by the photodetector of the sensor.