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Title: QUANTIFYING LANDSCAPE-SCALE MOVEMENT PATTERNS OF GLASSY-WINGED SHARPSHOOTER AND ITS NATURAL ENEMIES USING A NOVEL MARK-CAPTURE TECHNIQUE

Author
item Hagler, James
item Blackmer, Jacquelyn
item Henneberry, Thomas
item DAANE, KENT - U CA, BERKELEY
item Groves, Russell
item JONES, VINCENT - WA STATE UNIVERSITY

Submitted to: CDFA Pierce's Disease Control Program Research Symposium
Publication Type: Proceedings
Publication Acceptance Date: 12/12/2004
Publication Date: 12/12/2004
Citation: Hagler, J.R., Blackmer, J.L., Henneberry, T.J., Daane, K., Groves, R.L., Jones, V.P. 2004. Quantifying landscape-scale movement patterns of glassy-winged sharpshooter and its natural enemies using a novel mark-capture technique. CDFA Pierce's Disease Control Program Research Symposium. pp. 256-259

Interpretive Summary: Field cage studies were conducted to compare retention times between two inexpensive proteins, non fat dry milk (NFDM) and chicken egg whites, on glassy-wing sharpshooter (GWSS), Homalodisca coagulata and Hippodamia convergens. Each marker was applied to the insects by either directly spraying the insects with a conventional spraying device or by exposing the insects to pre-marked leaf tissue. Subsequently, the recaptured insects were analyzed by either an anti-NFDM or an antiegg white enzyme-linked immunosorbent assay (ELISA) to detect the presence of each respective marker. Data indicate that both protein markers were retained well on both insect species, regardless of the application method. Generally, the topical marking procedure yielded higher ELISA values than the insects marked by contact exposure; however, both methods were sufficient for marking almost 100% of each population for > 2 weeks.

Technical Abstract: Field cage studies were conducted to compare retention times between two inexpensive proteins, non fat dry milk (NFDM) and chicken egg whites, on glassy-wing sharpshooter (GWSS), Homalodisca coagulata and Hippodamia convergens. Each marker was applied to the insects by either directly spraying the insects with a conventional spraying device or by exposing the insects to pre-marked leaf tissue. Subsequently, the recaptured insects were analyzed by either an anti-NFDM or an antiegg white enzyme-linked immunosorbent assay (ELISA) to detect the presence of each respective marker. Data indicate that both protein markers were retained well on both insect species, regardless of the application method. Generally, the topical marking procedure yielded higher ELISA values than the insects marked by contact exposure; however, both methods were sufficient for marking almost 100% of each population for > 2 weeks.