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Submitted to: New Techniques and Applications for Lipid Analysis
Publication Type: Book / Chapter Publication Acceptance Date: 3/30/2005 Publication Date: 1/1/2006 Citation: Buyer, J.S. 2006. Fast gc for cellular fame analysis of bacteria. In: Mossoba, M.M., Brenna, J.T., McDonald, R.E., editors. Lipid Analysis and Lipidomics. New Techniques and Applications for Lipid Analysis. Champaign, IL: AOCS Press. p. 271-283. Interpretive Summary: . Technical Abstract: The cellular fatty acid composition of bacteria may be used to identify unknown bacterial isolates. A commercially available system (Microbial Identification System, MIDI, Newark, DE, USA) uses a four-step sample derivatization procedure. Sample processing time can be greatly reduced by using glass tubes in a 96-well format with multichannel pipetting. Gas chromatography run time can be reduced 6-fold by using a smaller column with higher gas flow rate and faster temperature programming. Taken together these modifications result in faster identifications and higher sample throughput. |
