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ARS Home » Southeast Area » Griffin, Georgia » Plant Genetic Resources Conservation Unit » Research » Publications at this Location » Publication #174795
Title: ISOLATION AND CHARACTERIZATION OF MICROSATELLITE MARKERS FROM MUSA BALBISIANA

Author
item BUHARIWALLA, H. - ICRISAT
item Jarret, Robert - Bob
item JAYASHREE, B. - ICRISAT
item CROUCH, J. - ICRISAT
item ORTIZ, R. - CIMMYT

Submitted to: Molecular Ecology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/1/2005
Publication Date: 4/1/2005
Citation: Buhariwalla, H.K., Jarret, R.L., Jayashree, B., Crouch, J.H., Ortiz, R. 2005. Isolation and characterization of microsatellite markers from musa balbisiana. Molecular Ecology. 5:327-330

Interpretive Summary: Genetic markers are commonly used to assess genetic diversity. Of the various types of genetic markers available for use in efforts to assess genetic diversity, microsatellites (also known as SSRs) have proven useful. This report describes the isolation and characterization of microsatellite markers for use to assess genetic diversity in Musa balbisiana, a relative of plantain.

Technical Abstract: This is the first report of targeted development of B genome microsatellite markers in Musa. A total of forty-four sequences with microsatellites were isolated from an enriched library of Musa balbisiana cultivar 'Tani', (BB genome). Of these, twenty-five were polymorphic when screened on 14 diverse diploid and triploid Musa accessions. The number of alleles detected by each marker ranged between 1 and 7. All twenty-five microsatellite markers generated amplification products in all species and genome complements. These new microsatellite markers fill an important gap for diversity assessment and linkage mapping studies in plantain (AAB) and cooking banana (AAB).