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Submitted to: Annual Eastern Fish Health Workshop
Publication Type: Abstract Only Publication Acceptance Date: 3/1/2004 Publication Date: 3/25/2004 Citation: Wiens, G.D. 2004. Identification of tumor necrosis factor (tnf) and tnf-receptor superfamily members expressed in rainbow trout (oncorhynchus mykiss). Annual Eastern Fish Health Workshop p 52. Interpretive Summary: Technical Abstract: Tumor necrosis factor (TNF) and its receptor are each prototype members of large gene superfamilies. Since the discovery of tumor necrosis factor and lymphotoxin ', 17 additional ligands and 29 cognate receptors have been identified in mammals. Many members of the TNF and TNF-receptor superfamilies play important roles in coordinating the proliferative and protective functions of pathogen-reactive cells. Some receptors function as decoy receptors as they lack a transmembrane domain and interfere with ligand signaling. These large superfamilies may exist in rainbow trout as two ligands, one receptor, and one decoy receptor have been identified. We searched a database of rainbow trout expressed sequence tags to identify additional genes that may belong in the ligand or receptor superfamilies. We have identified and sequenced two cDNA clones that have homology to TNF ligands and nine cDNA clones that share homology with TNF receptors. Of the putative receptor genes, four cDNA clones may be decoy receptors as they lack a predicted transmembrane domain. RNA expression analysis of these genes in Yersinia ruckeri bath vaccinated and challenged fish is underway. These data indicate that a number of TNF ligand and receptor superfamily members are expressed in rainbow trout and suggest that some of these genes may have arisen early in vertebrate evolution. |
