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ARS Home » Pacific West Area » Pullman, Washington » Plant Germplasm Introduction and Testing Research » Research » Publications at this Location » Publication #176082
Title: HIGH REGENERATION ABILITY OF BENZYLADENINE AND NAPTHALENE ACETIC ACID INDUCED CALLUS OF EASTERN HIBISCUS

Author
item Jenderek, Maria
item OLNEY, ARTHUR - CSU, FRESNO, CA

Submitted to: HortScience
Publication Type: Other
Publication Acceptance Date: 3/10/1999
Publication Date: N/A
Citation: Jenderek, M.M., Olney, A.J. 1999. High regeneration ability of benzyladenine and napthalene acetic acid induced callus of eastern hibiscus. Hortscience. V. 34(3). P. 458.

Interpretive Summary: NA. Abstract only.

Technical Abstract: The ability of Hibiscus syriacus explants to produce regenerable callus was investigated. Fragments of cotyledon, hypocotyl, and root were cultured on MS media supplemented with two different auxins (2,4-D and NAA, both 0.3 mg/L) and three different cytokinin (BA, 2iP and kinetin, all 0.1 mg/L). Plants were regenerated on McCown media with three different cytokinins at two different concentrations (0.1 and 1.0 mg/L). The biggest volume of callus was produced on medium containing 2,4-D/2iP (2,821 mm3/explant). The smallest mass of callus was induced on medium with NAA/kinetin (120 mm3/explant). On BA-supplemented media, the auxin type had no significant influence on the amount of callus produced. The highest number of shoots and leaves was produced on callus induced on NAA/BA supplemented media and regenerated on medium with 0.1 mg/L BA (4.2 shoots and 20 leaves/explant). Callus cultured on medium with 1.0 mg/L of BA produced significantly less shoots (2.4 shoots/explant). The lowest number of shoots was observed on callus originating from NAA/kinetin and NAA/2iP callus media grown on medium with kinetin or 2iP (both 1.0 mg/L). The highest number of roots was produced by cultures originating from NAA/BA callus medium grown on BA regeneration medium, irrespective of the cytokinin level. The longest shoots were observed on medium supplemented with 1.0 mg/L BA (18 mm). In this study, the best plant regeneration results were obtained for callus initiated on NAA/BA supplemented medium and regenerated on medium with 0.1 mg/L BA, however, the highest production of callus was observed on medium with 2,4-D/2iP.