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ARS Home » Pacific West Area » Pullman, Washington » Plant Germplasm Introduction and Testing Research » Research » Publications at this Location » Publication #176085
Title: RANDOM AMPLIFIED POLYMORPHIC DNA ANALYSIS OF GARLIC (ALLIUM SATIVUM L.) GERMPLASM COLLECTION

Author
item Jenderek, Maria
item SCHIERENBECK, KRISTINA - CSU, FRESNO, CA
item Hannan, Richard

Submitted to: HortScience
Publication Type: Other
Publication Acceptance Date: 5/1/1997
Publication Date: N/A
Citation: Jenderek, M.M., Schierenbeck, K., Hannan, R.M. 1997. Random amplified polymorphic dna analysis of garlic (Allium Sativum L.) germplasm collection. Hortscience. V. 32(3). P. 452.

Interpretive Summary: NA. Abstract only.

Technical Abstract: Maintenance of garlic (A. sativum) germplasm collections is based on year-to-year vegetative propagation of individual accessions. Several accessions are phenotypically similar, often originating from the same region of the world, but have been collected by different people at different times. These accessions are currently maintained as separate and unique samples, but may represent duplication in the collection. In order to identify genetic duplication in the USDA collection, 45 garlic Plant Introduction accessions from the garlic USDA germplasm collection were analyzed for RAPD marker polymorphism. The samples originated from 20 countries worldwide. RAPD bands were generated by 20 decamer primers, using 100-ng DNA template, and 38 PCR amplification samples, Polymorphism between accessions was defined as presence or absence of particular bands at given loci. However, a few distinguishing RAPD markers were established for selected accessions, identifying additional molecular markers to wholly assess the similarities or polymorphism of the garlic collection units is necessary.