Author
|
Submitted to: American Phytopathological Society Potomac Division Meeting
Publication Type: Abstract Only Publication Acceptance Date: 2/28/2005 Publication Date: 2/28/2005 Citation: Cooper, B. 2005. Identification of an unknown virus from infected plants using mass spectrometry. American Phytopathological Society Potomac Division Meeting. Interpretive Summary: Technical Abstract: We have identified a previously uncharacterized plant virus that is capable of infecting Nicotiana spp. and Arabidopsis thaliana. For diagnosis, we separated proteins from infected and noninfected plants on separate 2-D gels, excised the differentially expressed proteins, and analyzed them by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Experimental mass spectra were compared to virtual mass spectra generated from a comprehensive protein database via the SEQUEST algorithm, and the amino acid sequences of the peptides were inferred from the matches. This technique was suitable for identifying coat proteins of different viruses in infected plants and showed advantages over peptide mass fingerprinting and PCR for the characterization of unknown plant viruses. We believe that advances in mass spectrometry can be made to provide broad, reagent-independent diagnostic potential for diseases caused by viruses, bacteria and fungi. |
