Author
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LYNN, DWIGHT |
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Submitted to: Methods in Molecular Biology
Publication Type: Book / Chapter Publication Acceptance Date: 6/29/2005 Publication Date: N/A Citation: N/A Interpretive Summary: nsect cell lines are commonly used in research and product development by hundreds of government, university and industry facilities around the world. This is especially true in insect virus research, where they can be used for production of biopesticides or recombinant proteins. While more than 500 cell lines have been developed from 120 different insects, many important pest species are not represented. This chapter describes methods for developing new cell lines and is based on the author’s experience in developing dozens of cell lines from many different insect species and tissues. Technical Abstract: This chapter describes procedures for initiating new cell lines from lepidopteran larval tissues. The internal morphology is described along with methods for treating excised tissues and the primary cultures. Advice on culture medium and the tissues that will provide the best chance for new cell lines is discussed. |
