Skip to main content
ARS Home » Southeast Area » New Orleans, Louisiana » Southern Regional Research Center » Food and Feed Safety Research » Research » Publications at this Location » Publication #178350
Title: GENOME SEQUENCING AND ANALYSIS OF ASPERGILLUS ORYZAE

Author
item MACHIDA, MASAYUKI - AIST JAPAN
item ASAI, KIYOSHI - AIST JAPAN
item SANO, MOTOAKI - AIST JAPAN
item TANAKA, TOSHIHIRO - NATL RES INST OF BREWING
item KUMAGAI, TOSHITAKA - AIST JAPAN
item TERAI, GORO - AIST JAPAN
item KUSUMOTO, KEN-ICHI - NATL FOOD RESEARCH INST
item ARIMA, TOSHIHIDE - NATL RES INST OF BREWING
item AKITA, OSAMU - NATL RES INST OF BREWING
item KASHIWAGI, YUTAKA - NATL FOOD RESARCH INST
item ABE, KEIETSU - TOHOKU UNIVERSITY JAPAN
item GOMI, KATSUYA - TOHOKU UNIVERSITY JAPAN
item HORIUCHI, HIROYUKI - UNIVERSITY OF TOKYO
item KITAMOTO, KATSUHIKO - UNIVERSITY OF TOKYO
item KOBAYASHI, TETSUO - NAGOYA UNIVERSITY JAPAN
item TAKEUCHI, MICHIO - TOKYO UNIV OF AGR/TECH
item DENNING, DAVID - UNIVERSITY OF MANCHESTER
item GALAGAN, JAMES - BROAD INST OF MIT/HARVARD
item NIERMAN, WILLIAM - TIGR ROCKVILLE MD
item Yu, Jiujiang
item ARCHER, DAVID - UNIVERSITY OF NOTTINGHAM
item Bhatnagar, Deepak
item Cleveland, Thomas
item GOTOH, OSAMU - AIST JAPAN
item HORIKAWA, HIROSHI - NATL INST TECH/EVAL JAPAN
item HOSOYAMA, AKIRA - NATL INST TECH/EVAL JAPAN
item ICHINOMIYA, MASAYUKI - UNIVERSITY OF TOKYO
item IGARASHI, RIE - NATL INST TECH/EVAL JAPAN
item IWASHITA, KAZUHIRO - NATL RES INST OF BREWING
item JUVVADI, PRAVEEN RAO - UNIVERSITY OF TOKYO
item KATO, MASASHI - NAGOYA UNIVERSITY JAPAN
item KATO, YUMIKO - NATL INST TECH/EVAL JAPAN
item KIN, TAISHIN - AIST JAPAN
item KOKUBUN, AKIRA - NATL INST TECH/EVAL JAPAN
item MAEDA, HIROSHI - TOHOKU UNIVERSITY JAPAN
item MAEYAMA, NORIKO - NATL INST TECH/EVAL JAPAN
item MARUYAMA, JUN-ICHI - UNIVERSITY OF TOKYO
item NAGASAKI, HIDEKI - AIST JAPAN
item NAKAJIMA, TASUKU - TOHOKU UNIVERSITY JAPAN
item ODA, KEN - NATL RES INST OF BREWING
item OKADA, KINYA - AIST JAPAN
item PAULSEN, IAN - TIGR ROCKVILLE MD
item SAKAMOTO, KAZUTOSHI - NATL RES INST OF BREWING
item SAWANO, TOSHIHIKO - NATL INST TECH/EVAL JAPAN
item TAKAHASHI, MIKIO - NATL INST TECH/EVAL JAPAN
item TAKASE, KUMIKO - AIST JAPAN
item TERABAYASHI, TASUNORI - AIST JAPAN
item WORTMAN, JENNIFER - TIGR ROCKVILLE MD
item YAMADA, OSAMU - NATL RES INST OF BREWING
item YAMAGATA, OUHEI - TOHOKU UNIVERSITY JAPAN
item ANAZAWA, HIDEHARU - KYOWA HAKKO JAPAN
item HATA, YOJI - GEKKEIKAN SAKE CO JAPAN
item KOIDE, YOSHINAO - AMANO ENZYME JAPAN
item KOMORI, TAKASHI - INTEC WEB/GENOME INFORMAT
item KOYAMA, YASUJI - KIKKOMAN CORP JAPAN
item MINETOKI, TOSHITAKA - OZEKI CO JAPAN
item SUHARNAN, SIVASUNDARAM - AXIOHELIX JAPAN
item TANAKA, AKIMITSU - HIGETA SHOYU CO JAPAN
item ISONO, KATSUMI - NATL INST TECH/EVAL JAPAN
item KUHARA, SATORU - KYUSHU UNIVERSITY JAPAN
item OGASAWARA, NAOTAKE - NARA INST SCI/TECH JAPAN
item KIKUCHI, HISASHI - NATL INST TECH/EVAL JAPAN

Submitted to: Nature
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/1/2005
Publication Date: 12/22/2005
Citation: Machida, M., Asai, K., Sano, M., Tanaka, T., Kumagai, T., Terai, G., Kusumoto, K., Arima, T., Akita, O., Kashiwagi, Y., Abe, K., Gomi, K., Horiuchi, H., Kitamoto, K., Kobayashi, T., Takeuchi, M., Denning, D.W., Galagan, J.E., Nierman, W.C., Yu, J., Archer, D., Bhatnagar, D., Cleveland, T.E., Gotoh, O., Horikawa, H., Hosoyama, A., Ichinomiya, M., Igarashi, R., Iwashita, K., Juvvadi, P., Kato, M., Kato, Y., Kin, T., Kokubun, A., Maeda, H., Maeyama, N., Maruyama, J., Nagasaki, H., Nakajima, T., Oda, K., Okada, K., Paulsen, I., Sakamoto, K., Sawano, T., Takahashi, M., Takase, K., Terabayashi, T., Wortman, J., Yamada, O., Yamagata, O., Anazawa, H., Hata, Y., Koide, Y., Komori, T., Koyama, Y., Minetoki, T., Suharnan, S., Tanaka, A., Isono, K., Kuhara, S., Ogasawara, N., Kikuchi, H. 2005. Genome sequencing and analysis of Aspergillus oryzae. Nature. 438(7071):1157-1161.

Interpretive Summary: Aflatoxins are the most toxic and cancer-inducing compounds produced by certain fungi such as Aspergillus flavus and A. parasiticus. These compounds contaminate our food source such as corn, cotton, peanut, and tree nuts when the fungus infects crops. Understanding of the genetic and biochemical mechanisms of aflatoxin formation will provide valuable information on the control or elimination of aflatoxin contamination of food and feed. Aspergillus oryzae is an important industrial fungus used in the production of oriental fermented foods. A. oryzae is a close relative of A. flavus, which produces aflatoxins. The genome sequence of A. oryzae reveals a wealth of genetic information that scientists and researchers can compare with A. flavus and use to identify genes potentially involved in aflatoxin formation in A. flavus.

Technical Abstract: The genome of Aspergillus oryzae, an important industrial fungus used in the production of oriental fermented foods, such as soy sauce, miso, and sake, has been sequenced. The genome sequence reveals a wealth of genes encoding secreted enzymes. A comparison with the genome sequences of A. nidulans and A. fumigatus reveals that the A. oryzae genome is significantly larger than the other two genomes (6 Mb). In total, about 14,000 genes have been predicted, which is also 40 percent more than the other two genomes. This could be the result of acquisition during thousands of years of demastication and cultivation. Genes encoded by this acquired sequence are enriched for those predicted to be involved in metabolism, particularly in the synthesis of secondary metabolites. There is specific expansion of genes for secretary hydrolytic enzymes, amino acids metabolisms, and amino acids and sugar uptake transporters supporting the contention that A. oryzae is one of the ideal microorganisms for industrial fermentation.