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ARS Home » Southeast Area » Tifton, Georgia » Crop Protection and Management Research » Research » Publications at this Location » Publication #180685
Title: TRANSFERABILITY OF SOYBEAN SSR MARKERS IN PEANUT (ARACHIS HYPOGAEA L.)

Author
item HE, GUOHAO - TUSKEGEE UNIVERSITY
item WOULLARD, F - TUSKEGEE UNIVERSITY
item MARONG, I - TUSKEGEE UNIVERSITY
item Guo, Baozhu

Submitted to: Peanut Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/3/2005
Publication Date: 1/1/2006
Citation: He, G., Woullard, F.E., Marong, I., Guo, B.Z. 2006. Transferability of soybean SSR markers in peanut (Arachis hypogaea L.). Peanut Science. 33:22-28.

Interpretive Summary: Peanut is one of the major economically-important legume crops for protein and oil. While peanut offers numerous health benefits, it is one of the primary food allergens. Aflatoxin contamination of peanut is another important issue that threatens food/feed safety and human/animal health. Genomics plays an important role in altering peanuts to overcome many adverse conditions, as well as resolving serious health issues such as allergy and aflatoxin contamination. Simple sequence repeat (SSR) markers are a valuable tool in genetic mapping, genotyping, and marker-assisted selection in breeding. In peanut, SSR markers have been developed, however, they are still not fully exploited and developed compared to other crops such as soybean. Peanut genome research has less progress than other legumes because of insufficient genomic tools available. Thus, one of the pressing needs in peanut genome and genetic research is to take advantage of progress made in the well-characterized legumes for peanut genome researches. Searching transferable SSR marker developed from soybean is a cost-effective way to increase DNA markers for peanut research. In this study, four hundred thirty-two soybean SSR primer pairs were used to amplify peanut genomic DNAs extracted from four cultivated peanut lines. The result showed that 25% soybean SSR primer pairs tested in this study could amplify peanut genomic DNA. Among these transferable SSR markers, 28% have detected polymorphism among these peanut lines. These transferable markers will benefit peanut genome research by not only providing additional DNA markers in peanut, but also allowing comparative mapping to be possible between peanut and soybean.

Technical Abstract: Simple sequence repeats (SSRs) are useful DNA markers in plant genetic research. However, they are not fully exploited in peanut because of the high cost and labor intensity involved in their development. Many studies have showed that DNA markers could be transferable among related species due to the conservation regions in their genomes. The objective of this study was to investigate the transferability of soybean SSR markers to peanut because of the availability of a large number of soybean SSRs. Four hundred thirty-two soybean SSR primer pairs were used to amplify peanut genomic DNAs extracted from four cultivated peanut lines. The result showed that 25% soybean SSR primer pairs tested in this study could amplify peanut genomic DNA. Among these transferable SSR markers, 28% have detected polymorphism among these peanut lines. These transferable markers will benefit peanut genome research by not only providing additional DNA markers in peanut, but also allowing comparative mapping to be possible between peanut and soybean.