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Title: MOLECULAR ANALYSIS OF CAPSID PROTEIN OF HOMALODISCA COAGULATA VIRUS-1. NEW VIRUS FROM GLASSY-WINGED SHARPSHOOTER, HOMALODISCA COAGULATA (SAY) (HEMIPTERA: CICADELLIDAE)

Author
item Katsar, Catherine
item Hunter, Wayne
item CHAPARRO, JOSE - UNIVERSITY OF FLORIDA

Submitted to: Florida Entomological Society Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 7/22/2005
Publication Date: 7/24/2005
Citation: Katsar, C.S., Hunter, W.B., Chaparro, J.X. 2005. Molecular analysis of capsid protein of Homalodisca Coagulata Virus-1. New virus from Glassy-winged Sharpshooter, Homalodisca Coagulata (Say) (Hemiptera: Cicadellidae). 88th annual meeting of the Florida Entomological Society. July 24-27, 2005. Ft. Myers, FL. Paper No. DSP-12.

Interpretive Summary:

Technical Abstract: Capsid proteins have been shown to be a suitable target for phylogenetic studies in other insect viruses. A new insect infecting virus, isolated from the glassy-winged sharpshooter, GWSS, thus named, Homalodisca Coagulata Virus-1 (HoCV-1) demonstrated genetic relatedness to members within the Dicistroviridae, and appears to form a new clade within the newly recognized genus Cripavirus, “Cricket-Paralysis-like viruses”. Structural analysis of the HoCV-1 capsid protein indicated the presence of four domains with significant structural homology with previously established Cripavirus crystalline structures. HoCV-1 was found to increase sharpshooter mortality by ~20%, and was infective through microinjection, spraying, and bathing of insects with crude viral inoculum.