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ARS Home » Research » Publications at this Location » Publication #182374
Title: INCREASING THE DIVERSITY OF EST SEQUENCES FOR FRAGARIA

Author
item Slovin, Janet
item RABINOWICZ, PAUL - T.I.G.R.

Submitted to: American Society of Horticulture Science Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 7/6/2005
Publication Date: 7/15/2005
Citation: Slovin, J.P., Rabinowicz, P. 2005. Increasing the diversity of est sequences for fragaria. American Society of Horticulture Science Meeting.

Interpretive Summary:

Technical Abstract: The immediate objective of the proposed project is to increase the diversity of currently available EST sequences for the Rosaceae, in particular for strawberry, an agriculturally important member of this family. Approximately 35-40,000 sequences will be obtained representing 5 separate cDNA libraries from aseptically growing seedlings or greenhouse grown plants treated with a variety of abiotic stresses. These include cold, heat, salt, drought, and a combination of heat and drought. The resulting sequences will be assembled into clusters or Tentative Consensus (TC) sequences, annotated and maintained in the TIGR Gene Index Database (http://www.tigr.org/tdb/tgi/) as a Strawberry Gene Index. Sequences will also be made available through the GDR database. This work addresses the need for additional strawberry EST sequences and, by extending the range of publicly available sequences from the Rosaceae to those involved in stress responses, will provide a valuable resource to the entire Rosaceae community. The sequence data can be used by geneticists and breeders for developing markers for genetic mapping, and will also form the basis for developing gene-specific markers for loci potentially involved in abiotic stress resistance. The sequences will also provide a foundation for studies of stress response systems in the Rosaceae cDNa libraries will be constructed using the diploid strawberry, Fragaria vesca, which has a small genome size, 164Mb, and a cycle of 3.5-4 months from seed to seed. The genotype, PI551574 Hawaii 4, was chosen because it is day-neutral, produces runners, produces abundant seed, has a high germination rate without requiring cold treatment, and is easily transformed. Libraries will be constructed in an Invitrogen Gateway vector. This project is collaboration between the laboratory of Dr. Janet Slovin at the USDA in Beltsville MD, and Dr. Pablo Rabinowicz at The Institute for Genomic Research (TIGR) in Rockville, MD. The libraries will be constructed by Dr. Slovin from plant materials grown with the aid of an undergraduate greenhouse assistant. The sequencing will be performed at TIGR under the direction of Dr. Rabinowicz, who will also direct the development of the Strawberry Gene Index and train Dr. Slovin.