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Title: TWO BURSAL, B-CELL SUBPOPULATIONS WITH DIFFERENT FLOW CYTOMETRY PROFILES FOLLOWING IBDV INFECTION

Author
item PETKOV, DANIEL - UNIV OF GEORGIA-ATHENS
item LINNEMANN, ERICH - UNIV OF GEORGIA-ATHENS
item Kapczynski, Darrell
item SELLERS, HOLLY - UNIV OF GEORGIA-ATHENS

Submitted to: American Association of Avian Pathologists
Publication Type: Abstract Only
Publication Acceptance Date: 2/8/2005
Publication Date: 7/16/2005
Citation: Petkov, D.I., Linnemann, E.G., Kapczynski, D.R., Sellers, H.S. 2005. Two bursal, b-cell subpopulations with different flow cytometry profiles following ibdv infection. American Association of Avian Pathologists. p.59.

Interpretive Summary:

Technical Abstract: Infectious bursal disease virus (IBDV) is a cause of immunodeficiency in chickens. In the bursa of Fabricius, IBDV causes a reduction of surface IgM+ cells and follicular depletion. B-cell kinetics following IBDV vaccination and challenge was evaluated in the spleen and bursa with Bu1a/b, IgG, IgA, and IgM antibodies. Two B-cell subpopulations with different flow cytometry profile and kinetics were observed in the bursa. Subpopulation A, comprised of smaller IgM+ cells, was not affected and subpopulation B, the larger IgM+ cells, decreased after vaccination and challenge. The reduction of subpopulation B did not affect negatively the total serum IgA, IgG, and IgM immunoglobulins and IgG+ and IgA+ cells in the spleen. Subpopulation B, IgG+ cells in non-vaccinated group increased after challenge. Subpopulation A, IgA+ cells increased both after vaccination and challenge. Bursal B-cells were analyzed by Western blotting.