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ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Research Unit » Research » Publications at this Location » Publication #183487
Title: STRAWBERRY CHLOROTIC FLECK: A NEW CLOSTEROVIRUS ASSOCIATED WITH THE DISEASE

Author
item TZANETAKIS, I - OREGON STATE UNIVERSITY
item Martin, Robert

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 5/20/2005
Publication Date: 6/5/2005
Citation: Tzanetakis, I.E., Martin, R.R. 2005. Strawberry chlorotic fleck: a new closterovirus associated with the disease. Phytopathology. 95:S105.

Interpretive Summary:

Technical Abstract: Chlorotic fleck is a strawberry disease of unknown etiology for which the causal agent has been transmitted with the cotton aphid and speculated to be a virus. In the course of a project that involved the identification of causal agents for all strawberry diseases of unknown etiology, a chlorotic fleck infected plant was acquired from the National Clonal Germplasm Repository in Corvallis, Oregon. Double-stranded RNA was extracted and cloned. The plant was infected with both pallidosis agents, in addition to a novel virus, designated as Strawberry chlorotic fleck associated virus, with close homology to members of the genus Closterovirus, the aphid-borne members of the Closteroviridae. The majority of the genomic sequence obtained reveals a unique genome organization with features of both Beet yellows virus (BYV) and Citrus tristeza virus (CTV). Phylogenetic analysis indicates the close relationship of the virus, with CTV and BYV. A molecular detection test has been developed while transmission tests are under way to determine the aphid species able to transmit the virus.