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Title: SYNTHETIC HEXAPLOID WHEATS CAN EXPAND THE RANGE OF PUROINDOLINE HAPLOTYPES AND KERNEL TEXTURE IN TRITICUM AESTIVUM

Author
item GEDYE, K - WASHINGTON STATE UNIV
item Morris, Craig
item Bettge, Arthur
item Freston-Lenssen, Mishelle
item KING, G - WASHINGTON STATE UNIV

Submitted to: Cereal Conference Royal Australian Chemical Institute Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 9/27/2004
Publication Date: 12/1/2004
Citation: Gedye, K.R., Morris, C.F., Bettge, A.D., Freston, M.J., King, G.E. 2004. Synthetic hexaploid wheats can expand the range of puroindoline haplotypes and kernel texture in triticum aestivum. Cereal Conference Royal Australian Chemical Institute Proceedings. pp 220-222.

Interpretive Summary: The kernel softness of wheat is limited by the sole,‘wild-type’ puroindoline gene sequences contributed by wheat’s wild ancestor. “Synthetic” wheats provide greater and direct access to the wild ancestor gene pool, and thus access to a greater range in puroindoline gene sequences. Kernel softness varied among these synthetics. Eight unique puroindoline sequences were identified, one of which was identical to the soft ‘wild-type’ gene found in wheat. Four gene sequences of puroindoline were identical to sequences previously identified; three sequences were new. Compared to prior results with puroindoline sequence and kernel softness in wheat, none of the present puroindoline sequence polymorphisms produced a hard kernel.

Technical Abstract: The kernel softness of wheat is limited by the sole,‘wild-type’ puroindoline gene sequences contributed by wheat’s wild ancestor. Greater genetic variability in the puroindoline genes may expand the range of kernel texture in wheat. “Synthetic” wheats provide greater and direct access to the wild ancestor gene pool, and thus access to a greater range in puroindoline gene sequences. A set of synthetic wheat lines and assessed for kernel hardness. The two puroindoline genes were sequenced. Kernel softness varied among these synthetics from 2.6 to 40.9. Eight unique puroindoline sequences were identified, one of which was identical to the soft ‘wild-type’ gene found in wheat. Four gene sequences of puroindoline a were identical to sequences previously identified; three sequences were novel. Seven unique puroindoline b sequences were identified; three sequences were identical to those previously identified. Compared to prior results with puroindoline sequence and kernel softness in wheat, none of the present puroindoline sequence polymorphisms produced a hard kernel.