Author
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Kim, Sunran |
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Triplett, Barbara |
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Submitted to: National Cotton Council Beltwide Cotton Conference
Publication Type: Abstract Only Publication Acceptance Date: 11/10/2004 Publication Date: 1/4/2005 Citation: Kim, S., Triplett, B.A. 2005. Use of fluorescently labeled cellulose binding domain to monitor cotton fiber development. Proc. Beltwide Cotton Conf., New Orleans, LA, p. 2110. http://www.cotton. org/beltwide/proceedings/2005/pdfs/2910.pdf Interpretive Summary: Technical Abstract: Cellulose is the major component of the secondary wall in cotton fiber, and by the maturation stage, cotton fiber becomes almost pure cellulose. Although cotton fiber development is well synchronized in an ovary, it can differ between varieties and among plants from different environments. In this report, we used a protein fragment from a cellulase enzyme called the cellulose binding domain (CBD) to examine cellulose synthesis. First, CBD was covalently modified with a fluorescent probe called Texas Red. The fluorescent CBD was incubated for an hour with in vitro cultured cotton fibers of different ages: 10, 12, 13, 18, 26 and 40 day post anthesis (dpa). Fluorescence was detected in cotton fibers along the wall as early as 13 dpa, but not at earlier times. This method is rapid, highly specific for cellulose, and also suitable for comparing many samples at a time. The method will be useful for qualitative monitoring of the timing of cellulose biosynthesis under various growth conditions in vitro and in planta. |
