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ARS Home » Midwest Area » Urbana, Illinois » Soybean/maize Germplasm, Pathology, and Genetics Research » Research » Publications at this Location » Publication #184668
Title: INTERACTIONS BETWEEN SOYBEAN CYST NEMATODE AND FUSARIUM SOLANI F. SP. GLYCINES BASED ON GREENHOUSE FACTORIAL EXPERIMENTS

Author
item GAO, X - UNIVERSITY OF ILLINOIS
item JACKSON, T - UNIVERSITY OF NEBRASKA
item Hartman, Glen
item NIBLACK, T - UNIVERSITY OF ILLINOIS

Submitted to: Phytopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/14/2006
Publication Date: 12/1/2006
Citation: Gao, X., Jackson, T.A., Hartman, G.L., Niblack, T.L. 2006. Interactions between soybean cyst nematode and fusarium solani f. sp. glycines based on greenhouse factorial experiments. Phytopathology. 96:1409-1415.

Interpretive Summary: Both soybean cyst nematode (Heterodera glycines) and Fusarium solani f. sp. glycines are major pathogens of soybean and can cause significant yield losses. Both pathogens are soilborne and infect soybean roots. Studies on the relationship between H. glycines and F. solani f. sp. glycines have been investigated for nearly two decades, but the interaction is not clearly understood. In this study, greenhouse experiments were used to study the interactions between H. glycines and F. solani f. sp. glycines in factorial greenhouse experiments with different inoculum levels of both organisms on a soybean cultivar susceptible to both pathogens. Responses measured included root and shoot weights, H. glycines reproduction, area under the SDS progress curve, and fungal colonization. Both H. glycines and F. solani f. sp. glycines affected the growth of soybeans in an additive pattern. Reproduction of H. glycines was suppressed by high, but not low inoculum levels of F. solani f. sp. glycines. Molecular assays were used to quantify fungal infection of soybean roots by F. solani f. sp. glycines and indicated that the infection of soybean roots by H. glycines did not impact colonization of F. solani f. sp. glycines. This study is important to pathologist/nematologists that study interactions of pathogens.

Technical Abstract: Soybean cyst nematode, Heterodera glycines, and the fungus that causes sudden death syndrome of soybean (SDS), Fusarium solani f. sp. glycines, frequently co-infest soybean (Glycine max) fields. The interactions between H. glycines and F. solani f. sp. glycines were investigated in factorial greenhouse experiments with different inoculum levels of both organisms on a soybean cultivar susceptible to both pathogens. Responses measured included root and shoot fresh and dry weights, H. glycines reproduction, area under the SDS progress curve (AUDPC), and fungal colonization. Results of experiments conducted in two greenhouse environments were analyzed separately by 2-way, and 3-way analysis of variance (ANOVA) models that were developed that included the effect of greenhouse environment. Both H. glycines and F. solani f. sp. glycines affected the growth of soybeans in an additive pattern. Reproduction of H. glycines was suppressed (P=0.05) by high inoculum levels of F. solani f. sp. glycines, whereas the low level had no significant impact. Real-time PCR assays were used to quantify fungal infection of soybean roots by F. solani f. sp. glycines and indicated that the infection of soybean roots by H. glycines did not impact colonization of F. solani f. sp. glycines.