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Title: HAEMOPHILUS PARASUIS - NOVEL PROTEINS - HOPES FOR VACCINE?

Author
item MCVICKER, JERRY - IOWA STATE UNIVERSITY
item TABATABAI, LOUISA
item VAN SCHILFGAARDE, MURIEL - NETHERLANDS VACCINE INST

Submitted to: Swine Disease Conference Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 10/20/2004
Publication Date: 11/11/2004
Citation: McVicker, J.K., Tabatabai, L.B., van Schilfgaarde, M. 2004. Haemophilus parasuis - novel proteins - hopes for vaccine?. Proceedings of Swine Disease Conference for Swine Practitioners. p. 57-67.

Interpretive Summary:

Technical Abstract: The outer membrane protein P5 from Haemophilus influenzae has been shown to aid colonization in the human nasopharynx by binding to CD66 (CEA) on the surface of mucosal epithelial cells. Homologs to Haemophilus influenzae P5 and P2 colonization proteins were identified in Haemophilus parasuis and characterized by SDS-PAGE, IEF, and CEA binding. Using a CEA immunoblotting method, it was determined that the purified P5 protein did not bind CEA. However, CEA did bind to a P2 protein in all type strain serovars. Immunoblots with a monoclonal antibody to the H. influenzae P5 protein were also performed with H. parasuis reference strains. Results from the immunoblot indicate that the P5 and P2 proteins are present in all H. parasuis strains. Interestingly, the P2 protein was found to be 48 kDa in the virulent strains and 55 kDa in avirulent strains. The variable molecular weight of the P2 protein between the virulent and avirulent serovars suggests that it could potentially be used in disciminating between virulent and avirulent strains of H. parasuis.