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Title: INCREASING THE DIVERSITY OF EST SEQUENCES FOR FRAGARIA

Author
item Slovin, Janet
item RABINOWICZ, PABLO - INST. GENOMIC RESEARCH

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 10/11/2005
Publication Date: 1/14/2006
Citation: Slovin, J.P., Rabinowicz, P. 2006. Increasing the diversity of est sequences for fragaria. Meeting Abstract.

Interpretive Summary:

Technical Abstract: We have initiated a project to increase the diversity of EST sequences for the Rosaceae, in particular for strawberry, an agriculturally important member of this family. Rosaceae sequences representing genes expressed in stressed tissues are largely under-represented among the currently available cDNA sequences (mostly ESTs) in the public databases. Approximately 35-40,000 sequences will be obtained representing 5 separate cDNA libraries from aseptically growing seedlings or greenhouse grown plants treated with a variety of abiotic stresses. These include cold, heat, salt, drought, and a combination of heat and drought. The resulting sequences will be assembled into clusters or Tentative Consensus (TC) sequences, annotated and maintained in the TIGR Gene Index Database (http://www.tigr.org/tdb/tgi/) as a Strawberry Gene Index. Sequences will also be made available through the GDR database. We are using a diploid species of strawberry, Fragaria vesca, which is a useful model system for the family Rosaceae because it is a small plant with a small genome size (164Mb) and a cycle of 3.5-4 months from seed to seed. It is efficiently transformed and inbred lines are available. The genotype, PI551574 Hawaii 4, was chosen because it is day-neutral, produces runners, produces abundant seed, has a high germination rate without requiring cold treatment, and is easily transformed. Libraries are being constructed in Gateway vectors. The sequence data generated will form the basis for developing gene-specific markers for loci potentially involved in abiotic stress resistance, and will provide a foundation for studies of stress response systems in the Rosaceae.