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Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Proceedings Publication Acceptance Date: 1/14/2006 Publication Date: 1/14/2006 Citation: Frelichowski, J.E., Ulloa, M. 2006. Diversity assessment in cotton with new genetically mapped microsatellite markers. Plant and Animal Genome XIV Conference Proceedings. p. 136. Interpretive Summary: Technical Abstract: Cotton is one of the major crops produced worldwide which provides the world’s leading natural renewal fiber for the manufacture of textiles. In this day and age when most food crops are known to have narrow genetic variability and breeders continue to face tremendous pressure to use wild germplasm in their breeding programs, there is limited useful genetic information available from collections. Knowledge of variation in wild germplasm may enable collection and conservation of genetic diversity before the inevitable demise of wild germplasm due to loss of habitat. A set of 434 Gossypium hirsutum L. landraces from the USDA-ARS Cotton Germplasm Collection and 85 additional landraces from recent collections were evaluated phenotypically with common trait characters. In addition, the landraces are being assayed with new mapped microsatellite markers developed from ESTs, BAC-end sequences, and public markers selected from 23 of the 26 chromosomes of the cotton genome. The combination of morphological and molecular approaches will eventually produce a better description of the variation in collections, provide a framework for future data collection and description of germplasm, and may reveal relationships between the markers and morphological traits (i.e., linkage to genes). The use of anchored portable mapped molecular markers will characterize genome diversity and structural differences within or between cotton landraces and also illustrate how selection has shaped molecular and phenotypic variation in wild and cultivated cotton. |
