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Title: IN INTRO AND IN VIVO HOST RANGE STUDIES OF BACULOVIRUS THYSANOPLUSIA ORICHACEA NUCLEOPOLYHEDROVIRUS (THORMNPV)

Author
item WANG, LIHUA - MIAMI UNIV., OXFORD, OH
item Lynn, Dwight
item XUE, JIANLI - MIAMI UNIV., OXFORD,OH
item CHENG, XIAO-WEN - MIAMI UNIV., OXFORD,OH

Submitted to: Consortium for Plant Biotechnology Research
Publication Type: Abstract Only
Publication Acceptance Date: 11/4/2005
Publication Date: 11/4/2005
Citation: Wang, L., Lynn, D.E., Xue, J., Cheng, X. 2005. In intro and in vivo host range studies of baculovirus thysanoplusia orichacea nucleopolyhedrovirus (thormnpv) . Consortium for Plant Biotechnology Research.

Interpretive Summary:

Technical Abstract: To investigate the host range of Thysanoplusia orichacea nucleopolyhedrovirus (ThorMNPV), a green fluorescence protein gene (gfp) was inserted into the gp37 gene locus of ThorMNPV genome, and the recombinant virus (ThorMNPV-GFP) was obtained. ThorMNPV-GFP was tested in 29 insect cell lines from 14 insect species. Of these, 15 cell lines from 9 insect species showed green fluorescence with ThorMNPV-GFP indicating viral replication but at varying levels. The permissive lines include cells from Anticarsia gemmatalis, Ephestia kuenella, Heliothis virescens, Lymantria dispar, Mamestra brassicae, Spodoptera frugiperda, S. exigua, Christoneura fumiferana and Trichoplusia ni. Three of the insects from which the permissive cells were derived were not susceptible in previous host range studies: S. exigua, S. frugiperda, and A. gemmatalis. In another study, the mixed infection had over five times more cells infected by ThorMNPV-GFP (cells with fluorescence) than ThorMNPV-GFP alone in Sf21 cells. In an in vivo test, resistant larvae were fed with ThorMNPV-GFP OB and injected intrahemocoelically with budded virus of ThorMNPV-GFP. Replication of virus was observed in restricted tissues in all tested larvae by feeding but no mortality resulted although the viruses were vertically passed to the next generation. GFP could be detected in all stages of the insects. Injection of the budded viruses produced mortality in S. frugiperda (15%), S. exigua (20%) but not in Helicoverpa zea. We conclude the lack of mortality in resistant insects through per os inoculation of ThorMNPV is due to the limited release of budded virus from the midgut, slow replication and restricted tissue for infection.